2mpa
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2mpa.gif|left|200px]] | + | [[Image:2mpa.gif|left|200px]] |
| - | + | ||
| - | '''BACTERICIDAL ANTIBODY AGAINST NEISSERIA MENINGITIDIS''' | + | {{Structure |
| + | |PDB= 2mpa |SIZE=350|CAPTION= <scene name='initialview01'>2mpa</scene>, resolution 2.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CD:CADMIUM ION'>CD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''BACTERICIDAL ANTIBODY AGAINST NEISSERIA MENINGITIDIS''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2MPA is a [ | + | 2MPA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MPA OCA]. |
==Reference== | ==Reference== | ||
| - | Bactericidal antibody recognition of a PorA epitope of Neisseria meningitidis: crystal structure of a Fab fragment in complex with a fluorescein-conjugated peptide., van den Elsen JM, Herron JN, Hoogerhout P, Poolman JT, Boel E, Logtenberg T, Wilting J, Crommelin DJ, Kroon J, Gros P, Proteins. 1997 Sep;29(1):113-25. PMID:[http:// | + | Bactericidal antibody recognition of a PorA epitope of Neisseria meningitidis: crystal structure of a Fab fragment in complex with a fluorescein-conjugated peptide., van den Elsen JM, Herron JN, Hoogerhout P, Poolman JT, Boel E, Logtenberg T, Wilting J, Crommelin DJ, Kroon J, Gros P, Proteins. 1997 Sep;29(1):113-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9294871 9294871] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 20: | Line 29: | ||
[[Category: bactericidal antibody]] | [[Category: bactericidal antibody]] | ||
[[Category: complex (immunoglobulin/peptide)]] | [[Category: complex (immunoglobulin/peptide)]] | ||
| - | [[Category: epitope p1 16 of pora from neisseria | + | [[Category: epitope p1 16 of pora from neisseria meningitidi]] |
[[Category: murine immunoglobulin igg2a kappa]] | [[Category: murine immunoglobulin igg2a kappa]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:47:27 2008'' |
Revision as of 15:47, 20 March 2008
| |||||||
| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BACTERICIDAL ANTIBODY AGAINST NEISSERIA MENINGITIDIS
Overview
Class 1 outer membrane protein PorA of Neisseria meningitidis is a vaccine candidate against bacterial meningitis. Antibodies against PorA are able to induce complement-mediated bacterial killing and thereby play an important role in protection against meningococcal disease. Bactericidal antibodies are all directed against variable regions VR1 and VR2 of the PorA sequence, corresponding to loops 1 and 4 of a two-dimensional topology model of the porin with eight extracellular loops. We have determined the crystal structure to 2.6 A resolution of the Fab fragment of bactericidal antibody MN12H2 against meningococcal PorA in complex with a linear fluorescein-conjugated peptide TKDTNNNL derived from the VR2 sequence of sero-subtype P1.7,16 (residues 180-187) from meningococcal strain H44/76. The peptide folds deeply into the binding cavity of the Fab molecule in a type I beta-turn, with the minimal P1.16 epitope DTNNN virtually completely buried. The structure reveals H-bonds and van der Waals interactions with all minimal epitope residues and one essential salt bridge between Asp-182 of the peptide and His-31 of the MN12H2 light chain. The key components of the recognition of PorA epitope P1.16 by bactericidal antibody MN12H2 correspond well with available thermodynamic data from binding studies. Furthermore, they indicate the structural basis of an increased endemic incidence of infection by group B meningococci in England and Wales since 1981 associated with the occurrence of an Neisseria meningitidis escape mutant (strain-MC58). The observed three-dimensional conformation of the peptide provides a rationale for the development of a synthetic peptide vaccine against meningococcal disease.
About this Structure
2MPA is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Bactericidal antibody recognition of a PorA epitope of Neisseria meningitidis: crystal structure of a Fab fragment in complex with a fluorescein-conjugated peptide., van den Elsen JM, Herron JN, Hoogerhout P, Poolman JT, Boel E, Logtenberg T, Wilting J, Crommelin DJ, Kroon J, Gros P, Proteins. 1997 Sep;29(1):113-25. PMID:9294871
Page seeded by OCA on Thu Mar 20 17:47:27 2008
