2neo
From Proteopedia
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| - | [[Image:2neo.gif|left|200px]] | + | [[Image:2neo.gif|left|200px]] |
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| - | '''SOLUTION NMR STRUCTURE OF A TWO-BASE DNA BULGE COMPLEXED WITH AN ENEDIYNE CLEAVING ANALOG, 11 STRUCTURES''' | + | {{Structure |
| + | |PDB= 2neo |SIZE=350|CAPTION= <scene name='initialview01'>2neo</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NCS:SPIRO[[7-METHOXY-5-METHYL-1,2-DIHYDRO-NAPHTHALENE]-3,1'-[5-HYDROXY-9-[2-METHYLAMINO-2,6-DIDEOXYGALACTOPYRANOSYL-OXY]-5-(2-OXO-[1,3]DIOXOLAN-4-YL)-3A,5,9,9A-TETRAHYDRO-3H-1-OXA-CYCLOPENTA[A]-S-INDACEN-2-ONE]]'>NCS</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''SOLUTION NMR STRUCTURE OF A TWO-BASE DNA BULGE COMPLEXED WITH AN ENEDIYNE CLEAVING ANALOG, 11 STRUCTURES''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NEO is a [ | + | 2NEO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. This structure supersedes the now removed PDB entry 1NEO. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NEO OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of a two-base DNA bulge complexed with an enediyne cleaving analog., Stassinopoulos A, Ji J, Gao X, Goldberg IH, Science. 1996 Jun 28;272(5270):1943-6. PMID:[http:// | + | Solution structure of a two-base DNA bulge complexed with an enediyne cleaving analog., Stassinopoulos A, Ji J, Gao X, Goldberg IH, Science. 1996 Jun 28;272(5270):1943-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8658168 8658168] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Gao, X.]] | [[Category: Gao, X.]] | ||
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[[Category: Stassinopoulos, A.]] | [[Category: Stassinopoulos, A.]] | ||
[[Category: NCS]] | [[Category: NCS]] | ||
| - | [[Category: bulge binding | + | [[Category: bulge binding ligand]] |
[[Category: bulge dna]] | [[Category: bulge dna]] | ||
| - | [[Category: | + | [[Category: carbohydrate]] |
[[Category: deoxyribonucleic acid]] | [[Category: deoxyribonucleic acid]] | ||
[[Category: dna]] | [[Category: dna]] | ||
| - | [[Category: enediyne antibiotics antitumor | + | [[Category: enediyne antibiotics antitumor agent]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:47:46 2008'' |
Revision as of 15:47, 20 March 2008
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SOLUTION NMR STRUCTURE OF A TWO-BASE DNA BULGE COMPLEXED WITH AN ENEDIYNE CLEAVING ANALOG, 11 STRUCTURES
Overview
Nucleic acid bulges have been implicated in a number of biological processes and are specific cleavage targets for the enediyne antitumor antibiotic neocarzinostatin chromophore in a base-catalyzed, radical-mediated reaction. The solution structure of the complex between an analog of the bulge-specific cleaving species and an oligodeoxynucleotide containing a two-base bulge was elucidated by nuclear magnetic resonance. An unusual binding mode involves major groove recognition by the drug carbohydrate unit and tight fitting of the wedge-shaped drug in the triangular prism pocket formed by the two looped-out bulge bases and the neighboring base pairs. The two drug rings mimic helical DNA bases, complementing the bent DNA structure. The putative abstracting drug radical is 2.2 +/- 0.1 angstroms from the pro-S H5' of the target bulge nucleotide. This structure clarifies the mechanism of bulge recognition and cleavage by a drug and provides insight into the design of bulge-specific nucleic acid binding molecules.
About this Structure
2NEO is a Protein complex structure of sequences from [1]. This structure supersedes the now removed PDB entry 1NEO. Full crystallographic information is available from OCA.
Reference
Solution structure of a two-base DNA bulge complexed with an enediyne cleaving analog., Stassinopoulos A, Ji J, Gao X, Goldberg IH, Science. 1996 Jun 28;272(5270):1943-6. PMID:8658168
Page seeded by OCA on Thu Mar 20 17:47:46 2008
