2ns1

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[[Image:2ns1.jpg|left|200px]]<br /><applet load="2ns1" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2ns1.jpg|left|200px]]
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caption="2ns1, resolution 1.962&Aring;" />
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'''Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK'''<br />
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{{Structure
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|PDB= 2ns1 |SIZE=350|CAPTION= <scene name='initialview01'>2ns1</scene>, resolution 1.962&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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|ACTIVITY=
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|GENE= amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), glnK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2NS1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=BOG:'>BOG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS1 OCA].
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2NS1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS1 OCA].
==Reference==
==Reference==
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Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A., Gruswitz F, O'Connell J 3rd, Stroud RM, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):42-7. Epub 2006 Dec 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17190799 17190799]
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Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A., Gruswitz F, O'Connell J 3rd, Stroud RM, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):42-7. Epub 2006 Dec 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17190799 17190799]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: ammonia]]
[[Category: ammonia]]
[[Category: bog]]
[[Category: bog]]
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[[Category: center for structures of membrane proteins]]
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[[Category: center for structures of membrane protein]]
[[Category: channel]]
[[Category: channel]]
[[Category: csmp]]
[[Category: csmp]]
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[[Category: regulatory]]
[[Category: regulatory]]
[[Category: signal protein]]
[[Category: signal protein]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:10:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:50:19 2008''

Revision as of 15:50, 20 March 2008

Image:2ns1.jpg


PDB ID 2ns1

Drag the structure with the mouse to rotate
, resolution 1.962Å
Ligands: , and
Gene: amtB (Escherichia coli), glnK (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK


Overview

Ammonia conductance is highly regulated. A P(II) signal transduction protein, GlnK, is the final regulator of transmembrane ammonia conductance by the ammonia channel AmtB in Escherichia coli. The complex formed between AmtB and inhibitory GlnK at 1.96-A resolution shows that the trimeric channel is blocked directly by GlnK and how, in response to intracellular nitrogen status, the ability of GlnK to block the channel is regulated by uridylylation/deuridylylation at Y51. ATP and Mg(2+) augment the interaction of GlnK. The hydrolyzed product, adenosine 5'-diphosphate orients the surface of GlnK for AmtB blockade. 2-Oxoglutarate diminishes AmtB/GlnK association, and sites for 2-oxoglutarate are evaluated.

About this Structure

2NS1 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A., Gruswitz F, O'Connell J 3rd, Stroud RM, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):42-7. Epub 2006 Dec 26. PMID:17190799

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