2nv6

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[[Image:2nv6.gif|left|200px]]<br /><applet load="2nv6" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2nv6.gif|left|200px]]
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caption="2nv6, resolution 1.90&Aring;" />
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'''Mycobacterium tuberculosis InhA (S94A) bound with INH-NAD adduct'''<br />
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{{Structure
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|PDB= 2nv6 |SIZE=350|CAPTION= <scene name='initialview01'>2nv6</scene>, resolution 1.90&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZID:ISONICOTINIC-ACETYL-NICOTINAMIDE-ADENINE DINUCLEOTIDE'>ZID</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9]
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|GENE= inhA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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}}
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'''Mycobacterium tuberculosis InhA (S94A) bound with INH-NAD adduct'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2NV6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=ZID:'>ZID</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NV6 OCA].
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2NV6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NV6 OCA].
==Reference==
==Reference==
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Transfer of a point mutation in Mycobacterium tuberculosis inhA resolves the target of isoniazid., Vilcheze C, Wang F, Arai M, Hazbon MH, Colangeli R, Kremer L, Weisbrod TR, Alland D, Sacchettini JC, Jacobs WR Jr, Nat Med. 2006 Sep;12(9):1027-9. Epub 2006 Aug 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16906155 16906155]
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Transfer of a point mutation in Mycobacterium tuberculosis inhA resolves the target of isoniazid., Vilcheze C, Wang F, Arai M, Hazbon MH, Colangeli R, Kremer L, Weisbrod TR, Alland D, Sacchettini JC, Jacobs WR Jr, Nat Med. 2006 Sep;12(9):1027-9. Epub 2006 Aug 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16906155 16906155]
[[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
[[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: tuberculosis]]
[[Category: tuberculosis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:11:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:51:33 2008''

Revision as of 15:51, 20 March 2008

Image:2nv6.gif


PDB ID 2nv6

Drag the structure with the mouse to rotate
, resolution 1.90Å
Ligands:
Gene: inhA (Mycobacterium tuberculosis)
Activity: [acyl-carrier-protein_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number 1.3.1.9
Coordinates: save as pdb, mmCIF, xml



Mycobacterium tuberculosis InhA (S94A) bound with INH-NAD adduct


Overview

Isoniazid is one of the most effective antituberculosis drugs, yet its precise mechanism of action is still controversial. Using specialized linkage transduction, a single point mutation allele (S94A) within the putative target gene inhA was transferred in Mycobacterium tuberculosis. The inhA(S94A) allele was sufficient to confer clinically relevant levels of resistance to isoniazid killing and inhibition of mycolic acid biosynthesis. This resistance correlated with the decreased binding of the INH-NAD inhibitor to InhA, as shown by enzymatic and X-ray crystallographic analyses, and establishes InhA as the primary target of isoniazid action in M. tuberculosis.

About this Structure

2NV6 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Transfer of a point mutation in Mycobacterium tuberculosis inhA resolves the target of isoniazid., Vilcheze C, Wang F, Arai M, Hazbon MH, Colangeli R, Kremer L, Weisbrod TR, Alland D, Sacchettini JC, Jacobs WR Jr, Nat Med. 2006 Sep;12(9):1027-9. Epub 2006 Aug 13. PMID:16906155 [[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]

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