3wdz
From Proteopedia
| Line 1: | Line 1: | ||
{{STRUCTURE_3wdz| PDB=3wdz | SCENE= }} | {{STRUCTURE_3wdz| PDB=3wdz | SCENE= }} | ||
===Crystal Structure of Keap1 in Complex with phosphorylated p62=== | ===Crystal Structure of Keap1 in Complex with phosphorylated p62=== | ||
| + | {{ABSTRACT_PUBMED_24011591}} | ||
==Function== | ==Function== | ||
| Line 6: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | [[3wdz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | [[3wdz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WDZ OCA]. |
==Reference== | ==Reference== | ||
| - | <references group="xtra"/><references/> | + | <ref group="xtra">PMID:024011591</ref><references group="xtra"/><references/> |
| - | [[Category: | + | [[Category: Lk3 transgenic mice]] |
[[Category: Fukutomi, T.]] | [[Category: Fukutomi, T.]] | ||
[[Category: Komatsu, M.]] | [[Category: Komatsu, M.]] | ||
Revision as of 08:49, 25 December 2013
Contents |
Crystal Structure of Keap1 in Complex with phosphorylated p62
Template:ABSTRACT PUBMED 24011591
Function
[KEAP1_MOUSE] Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity).[1] [2] [SQSTM_MOUSE] Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures). Links ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Adapter that mediates the interaction between TRAF6 and CYLD.[3] [4]
About this Structure
3wdz is a 2 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA.
Reference
- Ichimura Y, Waguri S, Sou YS, Kageyama S, Hasegawa J, Ishimura R, Saito T, Yang Y, Kouno T, Fukutomi T, Hoshii T, Hirao A, Takagi K, Mizushima T, Motohashi H, Lee MS, Yoshimori T, Tanaka K, Yamamoto M, Komatsu M. Phosphorylation of p62 activates the Keap1-Nrf2 pathway during selective autophagy. Mol Cell. 2013 Sep 12;51(5):618-31. doi: 10.1016/j.molcel.2013.08.003. Epub 2013 , Sep 5. PMID:24011591 doi:http://dx.doi.org/10.1016/j.molcel.2013.08.003
- ↑ Itoh K, Wakabayashi N, Katoh Y, Ishii T, Igarashi K, Engel JD, Yamamoto M. Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain. Genes Dev. 1999 Jan 1;13(1):76-86. PMID:9887101
- ↑ McMahon M, Itoh K, Yamamoto M, Hayes JD. Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expression. J Biol Chem. 2003 Jun 13;278(24):21592-600. Epub 2003 Apr 7. PMID:12682069 doi:10.1074/jbc.M300931200
- ↑ Duran A, Serrano M, Leitges M, Flores JM, Picard S, Brown JP, Moscat J, Diaz-Meco MT. The atypical PKC-interacting protein p62 is an important mediator of RANK-activated osteoclastogenesis. Dev Cell. 2004 Feb;6(2):303-9. PMID:14960283
- ↑ Jin W, Chang M, Paul EM, Babu G, Lee AJ, Reiley W, Wright A, Zhang M, You J, Sun SC. Deubiquitinating enzyme CYLD negatively regulates RANK signaling and osteoclastogenesis in mice. J Clin Invest. 2008 May;118(5):1858-66. doi: 10.1172/JCI34257. PMID:18382763 doi:10.1172/JCI34257
