2nw0
From Proteopedia
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| - | [[Image:2nw0.gif|left|200px]] | + | [[Image:2nw0.gif|left|200px]] |
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| - | '''Crystal structure of a lysin''' | + | {{Structure |
| + | |PDB= 2nw0 |SIZE=350|CAPTION= <scene name='initialview01'>2nw0</scene>, resolution 1.600Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACT:ACETATE ION'>ACT</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | ||
| + | |GENE= PlyB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38018 unidentified phage]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a lysin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NW0 is a [ | + | 2NW0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Unidentified_phage Unidentified phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NW0 OCA]. |
==Reference== | ==Reference== | ||
| - | The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracis., Porter CJ, Schuch R, Pelzek AJ, Buckle AM, McGowan S, Wilce MC, Rossjohn J, Russell R, Nelson D, Fischetti VA, Whisstock JC, J Mol Biol. 2007 Feb 16;366(2):540-50. Epub 2006 Nov 18. PMID:[http:// | + | The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracis., Porter CJ, Schuch R, Pelzek AJ, Buckle AM, McGowan S, Wilce MC, Rossjohn J, Russell R, Nelson D, Fischetti VA, Whisstock JC, J Mol Biol. 2007 Feb 16;366(2):540-50. Epub 2006 Nov 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17182056 17182056] |
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: beta barrel]] | [[Category: beta barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:51:55 2008'' |
Revision as of 15:51, 20 March 2008
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| , resolution 1.600Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | PlyB (unidentified phage) | ||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a lysin
Overview
Lysins are peptidoglycan hydrolases that are produced by bacteriophage and act to lyse the bacterial host cell wall during progeny phage release. Here, we describe the structure and function of a novel bacteriophage-derived lysin, PlyB, which displays potent lytic activity against the Bacillus anthracis-like strain ATCC 4342. This molecule comprises an N-terminal catalytic domain (PlyB(cat)) and a C-terminal bacterial SH3-like domain, SH3b. It is shown that both domains are required for effective catalytic activity against ATCC 4342. Further, PlyB has specific activity comparable to the phage lysin PlyG, an amidase being developed as a therapeutic against anthrax. In contrast to PlyG, however, the 1.6 A X-ray crystal structure of PlyB(cat) reveals that the catalytic domain adopts the glycosyl hydrolase (GH)-25, rather than phage T7 lysozyme-like fold. PlyB therefore represents a new class of anthrax lysin and a new defensive tool in the armament against anthrax-mediated bioterrorism.
About this Structure
2NW0 is a Protein complex structure of sequences from Unidentified phage. Full crystallographic information is available from OCA.
Reference
The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracis., Porter CJ, Schuch R, Pelzek AJ, Buckle AM, McGowan S, Wilce MC, Rossjohn J, Russell R, Nelson D, Fischetti VA, Whisstock JC, J Mol Biol. 2007 Feb 16;366(2):540-50. Epub 2006 Nov 18. PMID:17182056
Page seeded by OCA on Thu Mar 20 17:51:55 2008
