1dpo

From Proteopedia

Revision as of 06:37, 30 October 2007

STRUCTURE OF RAT TRYPSIN

Overview

The structure of a rat trypsin mutant [S195C] at a temperature of 120 K, has been refined to a crystallographic R factor of 17.4% between 12.0 and, 1.59 A and is compared with the structure of the D102N mutant at 295 K. A, reduction in the unit cell dimensions in going from room temperature to, low temperature is accompanied by a decrease in molecular surface area and, radius of gyration. The overall structure remains similar to that at room, temperature. The attainable resolution appears to be improved due to the, decrease in the fall off of intensities with resolution [reduction of the, temperature factor]. This decreases the uncertainty in the atomic, positions and allows the localization of more protein atoms and solvent, molecules in the low temperature map. The largest differences ... [(full description)]

About this Structure

1DPO is a [Single protein] structure of sequence from [Rattus rattus] with CA, SO4 and BEN as [ligands]. Active as [Hydrolase], with EC number [3.4.21.4]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].

Reference

1.59 A structure of trypsin at 120 K: comparison of low temperature and room temperature structures., Earnest T, Fauman E, Craik CS, Stroud R, Proteins. 1991;10(3):171-87. PMID:1881877

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