2o9f
From Proteopedia
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| - | [[Image:2o9f.jpg|left|200px]] | + | [[Image:2o9f.jpg|left|200px]] |
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| - | '''Crystal Structure of AqpZ mutant L170C''' | + | {{Structure |
| + | |PDB= 2o9f |SIZE=350|CAPTION= <scene name='initialview01'>2o9f</scene>, resolution 2.550Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= aqpZ, bniP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of AqpZ mutant L170C''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2O9F is a [ | + | 2O9F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9F OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of aquaporin inhibition by mercury., Savage DF, Stroud RM, J Mol Biol. 2007 May 4;368(3):607-17. Epub 2007 Mar 2. PMID:[http:// | + | Structural basis of aquaporin inhibition by mercury., Savage DF, Stroud RM, J Mol Biol. 2007 May 4;368(3):607-17. Epub 2007 Mar 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17376483 17376483] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: integral membrane protein]] | [[Category: integral membrane protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:56:50 2008'' |
Revision as of 15:56, 20 March 2008
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| , resolution 2.550Å | |||||||
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| Gene: | aqpZ, bniP (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of AqpZ mutant L170C
Overview
The aquaporin family of channels was defined based on the inhibition of water transport by mercurial compounds. Despite the important role of mercurials, little is known about the structural changes involved upon mercury binding leading to channel inhibition. To elucidate the mechanism we designed a mutant, T183C, of aquaporin Z (AqpZ) patterned after the known mercury-sensitive site of aquaporin 1 (AQP1) and determined the X-ray crystal structures of the unbound and mercury blocked states. Superposition of the two structures shows no conformational rearrangement upon mercury binding. In the blocked structure, there are two mercury sites, one bound to Cys183 and occluding the pore, and a second, also bound to the same cysteine but found buried in an interstitial cavity. To test the mechanism of blockade we designed a different mutant, L170C, to produce a more effective mercury block at the pore site. In a dose-response inhibition study, this mutant was 20 times more sensitive to mercury than wild-type AqpZ and four times more sensitive than T183C. The X-ray structure of L170C shows four mercury atoms at, or near, the pore site defined in the T183C structure and no structural change upon mercury binding. Thus, we elucidate a steric inhibition mechanism for this important class of channels by mercury.
About this Structure
2O9F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis of aquaporin inhibition by mercury., Savage DF, Stroud RM, J Mol Biol. 2007 May 4;368(3):607-17. Epub 2007 Mar 2. PMID:17376483
Page seeded by OCA on Thu Mar 20 17:56:50 2008
