2oam
From Proteopedia
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| - | [[Image:2oam.gif|left|200px]] | + | [[Image:2oam.gif|left|200px]] |
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| - | '''Apo RebH from Lechevalieria aerocolonigenes''' | + | {{Structure |
| + | |PDB= 2oam |SIZE=350|CAPTION= <scene name='initialview01'>2oam</scene>, resolution 2.300Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= rbmJ, rebH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes]) | ||
| + | }} | ||
| + | |||
| + | '''Apo RebH from Lechevalieria aerocolonigenes''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OAM is a [ | + | 2OAM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAM OCA]. |
==Reference== | ==Reference== | ||
| - | Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases., Yeh E, Blasiak LC, Koglin A, Drennan CL, Walsh CT, Biochemistry. 2007 Feb 6;46(5):1284-92. PMID:[http:// | + | Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases., Yeh E, Blasiak LC, Koglin A, Drennan CL, Walsh CT, Biochemistry. 2007 Feb 6;46(5):1284-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17260957 17260957] |
[[Category: Lechevalieria aerocolonigenes]] | [[Category: Lechevalieria aerocolonigenes]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tryptophan-7-halogenase]] | [[Category: tryptophan-7-halogenase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:57:11 2008'' |
Revision as of 15:57, 20 March 2008
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| , resolution 2.300Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | rbmJ, rebH (Lechevalieria aerocolonigenes) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Apo RebH from Lechevalieria aerocolonigenes
Overview
The flavin-dependent halogenase RebH catalyzes the formation of 7-chlorotryptophan as the initial step in the biosynthesis of antitumor agent rebeccamycin. The reaction of FADH2, Cl-, and O2 in the active site generates the powerful oxidant HOCl, which was presumed to carry out the chlorination reaction. Herein, we demonstrate the formation of a long-lived chlorinating intermediate (t1/2 = 63 h at 4 degrees C) when RebH, FADH2, Cl-, and O2 react in the absence of substrate tryptophan. This intermediate remained on the enzyme after removal of FAD and transferred chlorine to tryptophan with kinetically competent rates. The identity of this intermediate is suggested by the X-ray crystal structure of RebH, which revealed an active site Lys79 located in a central position between flavin and tryptophan binding sites and just 4.1 A above C7 of tryptophan. The chlorinating species is proposed to be a Lys-epsilonNH-Cl (lysine chloramine) from reaction of enzyme-generated HOCl with the active site Lys79. This covalent enzyme chloramine likely plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes.
About this Structure
2OAM is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA.
Reference
Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases., Yeh E, Blasiak LC, Koglin A, Drennan CL, Walsh CT, Biochemistry. 2007 Feb 6;46(5):1284-92. PMID:17260957
Page seeded by OCA on Thu Mar 20 17:57:11 2008
