2ofq

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[[Image:2ofq.gif|left|200px]]<br /><applet load="2ofq" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2ofq.gif|left|200px]]
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caption="2ofq" />
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'''NMR Solution Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system'''<br />
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{{Structure
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|PDB= 2ofq |SIZE=350|CAPTION= <scene name='initialview01'>2ofq</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= traO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=179945 IncN plasmid R46]), traN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=179945 IncN plasmid R46])
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}}
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'''NMR Solution Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2OFQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Incn_plasmid_r46 Incn plasmid r46]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFQ OCA].
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2OFQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Incn_plasmid_r46 Incn plasmid r46]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFQ OCA].
==Reference==
==Reference==
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NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system., Bayliss R, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll PC, Waksman G, Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1673-8. Epub 2007 Jan 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17244707 17244707]
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NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system., Bayliss R, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll PC, Waksman G, Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1673-8. Epub 2007 Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17244707 17244707]
[[Category: Incn plasmid r46]]
[[Category: Incn plasmid r46]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: trao]]
[[Category: trao]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:18:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:59:05 2008''

Revision as of 15:59, 20 March 2008

Image:2ofq.gif


PDB ID 2ofq

Drag the structure with the mouse to rotate
Gene: traO (IncN plasmid R46), traN (IncN plasmid R46)
Coordinates: save as pdb, mmCIF, xml



NMR Solution Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system


Overview

Type IV secretion (T4S) systems translocate DNA and protein effectors through the double membrane of Gram-negative bacteria. The paradigmatic T4S system in Agrobacterium tumefaciens is assembled from 11 VirB subunits and VirD4. Two subunits, VirB9 and VirB7, form an important stabilizing complex in the outer membrane. We describe here the NMR structure of a complex between the C-terminal domain of the VirB9 homolog TraO (TraO(CT)), bound to VirB7-like TraN from plasmid pKM101. TraO(CT) forms a beta-sandwich around which TraN winds. Structure-based mutations in VirB7 and VirB9 of A. tumefaciens show that the heterodimer interface is conserved. Opposite this interface, the TraO structure shows a protruding three-stranded beta-appendage, and here, we supply evidence that the corresponding region of VirB9 of A. tumefaciens inserts in the membrane and protrudes extracellularly. This complex structure elucidates the molecular basis for the interaction between two essential components of a T4S system.

About this Structure

2OFQ is a Protein complex structure of sequences from Incn plasmid r46. Full crystallographic information is available from OCA.

Reference

NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system., Bayliss R, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll PC, Waksman G, Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1673-8. Epub 2007 Jan 23. PMID:17244707

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