2opc
From Proteopedia
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| - | [[Image:2opc.gif|left|200px]] | + | [[Image:2opc.gif|left|200px]] |
| - | + | ||
| - | '''Structure of Melampsora lini avirulence protein, AvrL567-A''' | + | {{Structure |
| + | |PDB= 2opc |SIZE=350|CAPTION= <scene name='initialview01'>2opc</scene>, resolution 1.43Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene> and <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= AvrL567-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5261 Melampsora lini]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of Melampsora lini avirulence protein, AvrL567-A''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OPC is a [ | + | 2OPC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Melampsora_lini Melampsora lini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OPC OCA]. |
==Reference== | ==Reference== | ||
| - | The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:[http:// | + | The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17329816 17329816] |
[[Category: Melampsora lini]] | [[Category: Melampsora lini]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: single-wavelength anomalous dispersion (sad)]] | [[Category: single-wavelength anomalous dispersion (sad)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:02:44 2008'' |
Revision as of 16:02, 20 March 2008
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| , resolution 1.43Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | AvrL567-A (Melampsora lini) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Melampsora lini avirulence protein, AvrL567-A
Overview
Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available.
About this Structure
2OPC is a Single protein structure of sequence from Melampsora lini. Full crystallographic information is available from OCA.
Reference
The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:17329816
Page seeded by OCA on Thu Mar 20 18:02:44 2008
