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4isq

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{{STRUCTURE_4isq| PDB=4isq | SCENE= }}
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==Binding domain of Botulinum neurotoxin DC in complex with human synaptotagmin I==
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===Binding domain of Botulinum neurotoxin DC in complex with human synaptotagmin I===
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<StructureSection load='4isq' size='340' side='right' caption='[[4isq]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23932591}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4isq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ISQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4isr|4isr]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4isq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4isq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4isq RCSB], [http://www.ebi.ac.uk/pdbsum/4isq PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Botulinum neurotoxins (BoNTs) can cause paralysis at exceptionally low concentrations and include seven serotypes (BoNT/A-G). The chimeric BoNT/DC toxin has a receptor binding domain similar to the same region in BoNT/C. However, BoNT/DC does not share protein receptor with BoNT/C. Instead, it shares synaptotagmin (Syt) I and II as receptors with BoNT/B, despite their low sequence similarity. Here, we present the crystal structures of the binding domain of BoNT/DC in complex with the recognition domains of its protein receptors, Syt-I and Syt-II. The structures reveal that BoNT/DC possesses a Syt binding site, distinct from the established Syt-II binding site in BoNT/B. Structure-based mutagenesis further shows that hydrophobic interactions play a key role in Syt binding. The structures suggest that the BoNT/DC ganglioside binding sites are independent of the protein receptor binding site. Our results reveal the remarkable versatility in the receptor recognition of the BoNTs.
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==Function==
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Crystal Structures of Botulinum Neurotoxin DC in Complex with Its Protein Receptors Synaptotagmin I and II.,Berntsson RP, Peng L, Svensson LM, Dong M, Stenmark P Structure. 2013 Sep 3;21(9):1602-11. doi: 10.1016/j.str.2013.06.026. Epub 2013, Aug 8. PMID:23932591<ref>PMID:23932591</ref>
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[[http://www.uniprot.org/uniprot/SYT1_HUMAN SYT1_HUMAN]] May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4isq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISQ OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023932591</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Clostridium botulinum]]
[[Category: Clostridium botulinum]]
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[[Category: Berntsson, R P.A.]]
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[[Category: Berntsson, R P.A]]
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[[Category: Dong, M.]]
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[[Category: Dong, M]]
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[[Category: Peng, L.]]
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[[Category: Peng, L]]
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[[Category: Stenmark, P.]]
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[[Category: Stenmark, P]]
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[[Category: Svensson, L M.]]
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[[Category: Svensson, L M]]
[[Category: Membrane binding]]
[[Category: Membrane binding]]
[[Category: Synaptotagmin and ganglioside binding]]
[[Category: Synaptotagmin and ganglioside binding]]
[[Category: Toxin]]
[[Category: Toxin]]

Revision as of 12:45, 21 December 2014

Binding domain of Botulinum neurotoxin DC in complex with human synaptotagmin I

4isq, resolution 2.65Å

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