4kbm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_4kbm| PDB=4kbm | SCENE= }}
+
==Structure of the Mtb CarD/RNAP Beta subunit B1-B2 domains complex==
-
===Structure of the Mtb CarD/RNAP Beta subunit B1-B2 domains complex===
+
<StructureSection load='4kbm' size='340' side='right' caption='[[4kbm]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_24055315}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4kbm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KBM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KBM FirstGlance]. <br>
 +
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kbj|4kbj]]</td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT0695, MTCI376.08c, rpoB, Rv0667 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis]), carD, MT3689, Rv3583c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
 +
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kbm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kbm RCSB], [http://www.ebi.ac.uk/pdbsum/4kbm PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
CarD from Mycobacterium tuberculosis (Mtb) is an essential protein shown to be involved in stringent response through downregulation of rRNA and ribosomal protein genes. CarD interacts with the beta-subunit of RNAP and this interaction is vital for Mtb's survival during the persistent infection state. We have determined the crystal structure of CarD in complex with the RNAP beta-subunit beta1 and beta2 domains at 2.1 A resolution. The structure reveals the molecular basis of CarD/RNAP interaction, providing a basis to further our understanding of RNAP regulation by CarD. The structural fold of the CarD N-terminal domain is conserved in RNAP interacting proteins such as TRCF-RID and CdnL, and displays similar interactions to the predicted homology model based on the TRCF/RNAP beta1 structure. Interestingly, the structure of the C-terminal domain, which is required for complete CarD function in vivo, represents a distinct DNA-binding fold.
-
==Function==
+
Structure of the Mtb CarD/RNAP beta-Lobes Complex Reveals the Molecular Basis of Interaction and Presents a Distinct DNA-Binding Domain for Mtb CarD.,Gulten G, Sacchettini JC Structure. 2013 Sep 17. pii: S0969-2126(13)00306-7. doi:, 10.1016/j.str.2013.08.014. PMID:24055315<ref>PMID:24055315</ref>
-
[[http://www.uniprot.org/uniprot/RPOB_MYCTU RPOB_MYCTU]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01321] [[http://www.uniprot.org/uniprot/CARD_MYCTU CARD_MYCTU]] Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.<ref>PMID:19596241</ref> <ref>PMID:22904282</ref>
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[4kbm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KBM OCA].
+
</div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:024055315</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: DNA-directed RNA polymerase]]
[[Category: DNA-directed RNA polymerase]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
-
[[Category: Gulten, G.]]
+
[[Category: Gulten, G]]
-
[[Category: Sacchettini, J C.]]
+
[[Category: Sacchettini, J C]]
-
[[Category: TBSGC, TB Structural Genomics Consortium.]]
+
[[Category: Structural genomic]]
[[Category: Card]]
[[Category: Card]]
[[Category: Dna]]
[[Category: Dna]]
Line 21: Line 30:
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
[[Category: Sigma factor]]
[[Category: Sigma factor]]
-
[[Category: Structural genomic]]
 
-
[[Category: Tb structural genomics consortium]]
 
[[Category: Tbsgc]]
[[Category: Tbsgc]]
[[Category: Transcription regulator]]
[[Category: Transcription regulator]]
[[Category: Transferase-transcription complex]]
[[Category: Transferase-transcription complex]]
[[Category: Tudor]]
[[Category: Tudor]]

Revision as of 15:29, 21 December 2014

Structure of the Mtb CarD/RNAP Beta subunit B1-B2 domains complex

4kbm, resolution 2.11Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4kbm"
Personal tools