This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3wfz
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Crystal structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase C236Y Mutant== | |
| - | + | <StructureSection load='3wfz' size='340' side='right' caption='[[3wfz]], [[Resolution|resolution]] 2.60Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3wfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifidobacterium_longum_subsp._longum_jcm_1217 Bifidobacterium longum subsp. longum jcm 1217]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WFZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zuu|2zuu]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BLLJ_1623, lnpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=565042 Bifidobacterium longum subsp. longum JCM 1217])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,3-beta-galactosyl-N-acetylhexosamine_phosphorylase 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.211 2.4.1.211] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wfz RCSB], [http://www.ebi.ac.uk/pdbsum/3wfz PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Galacto-N-biose/lacto-N-biose I phosphorylase (GLNBP) is the key enzyme in the enzymatic production of lacto-N-biose I. For the purpose of industrial use, we improved the thermostability of GLNBP by evolutionary engineering in which five substitutions in the amino acid sequence were selected from a random mutagenesis GLNBP library constructed using error-prone polymerase chain reaction. Among them, C236Y and D576V mutants showed considerably improved thermostability. Structural analysis of C236Y revealed that the hydroxyl group of Tyr236 forms a hydrogen bond with the carboxyl group of E319. The C236Y and D576V mutations together contributed to the thermostability. The C236Y/D576V mutant exhibited 20 degrees C higher thermostability than the wild type. | ||
| - | + | Directed evolution to enhance thermostability of galacto-N-biose/lacto-N-biose I phosphorylase.,Koyama Y, Hidaka M, Nishimoto M, Kitaoka M Protein Eng Des Sel. 2013 Sep 24. PMID:24065834<ref>PMID:24065834</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase]] | [[Category: 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase]] | ||
[[Category: Bifidobacterium longum subsp. longum jcm 1217]] | [[Category: Bifidobacterium longum subsp. longum jcm 1217]] | ||
| - | [[Category: Hidaka, M | + | [[Category: Hidaka, M]] |
| - | [[Category: Kawakami, M | + | [[Category: Kawakami, M]] |
| - | [[Category: Kitaoka, M | + | [[Category: Kitaoka, M]] |
| - | [[Category: Koyama, Y | + | [[Category: Koyama, Y]] |
| - | [[Category: Nishimoto, M | + | [[Category: Nishimoto, M]] |
[[Category: Beta-alpha-barrel]] | [[Category: Beta-alpha-barrel]] | ||
[[Category: Phosphorylase]] | [[Category: Phosphorylase]] | ||
[[Category: Tim barrel]] | [[Category: Tim barrel]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 09:18, 21 December 2014
Crystal structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase C236Y Mutant
| |||||||||||
