4kt5
From Proteopedia
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| - | + | ==Structure of GrlR-GrlA complex== | |
| - | === | + | <StructureSection load='4kt5' size='340' side='right' caption='[[4kt5]], [[Resolution|resolution]] 2.70Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4kt5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KT5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">grlR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7]), grlA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kt5 RCSB], [http://www.ebi.ac.uk/pdbsum/4kt5 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The locus of enterocyte effacement (LEE) is essential for virulence of enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC). The 41 genes of the LEE encode type III secretion system proteins and three associated regulators: Ler, GrlA and GrlR. Ler is a positive regulator for most of the LEE operons, including grlRA. GrlA controls the expression of ler, ehxCABD and flhDC operons. GrlR binds to GrlA and suppresses its function. Here we report the crystal structure of GrlR-GrlADelta (aa 1-106) complex (2:1) and its functional characterization. We show that GrlR interacts with the Helix-Turn-Helix motif of GrlA. Moreover, GrlA binds to the promoter DNA fragments of ler, ehxCABD and flhDC, and GrlR outcompetes with these promoter DNA sequences for the Helix-Turn-Helix motif of GrlA. These findings provide mechanistic insight into a regulatory module for the virulence of EPEC and EHEC, two important pathogens that cause devastating diseases. | ||
| - | + | Structure of GrlR-GrlA complex that prevents GrlA activation of virulence genes.,Padavannil A, Jobichen C, Mills E, Velazquez-Campoy A, Li M, Leung KY, Mok YK, Rosenshine I, Sivaraman J Nat Commun. 2013 Oct 4;4:2546. doi: 10.1038/ncomms3546. PMID:24092262<ref>PMID:24092262</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Escherichia coli o157:h7]] | [[Category: Escherichia coli o157:h7]] | ||
| - | [[Category: Jobichen, C | + | [[Category: Jobichen, C]] |
| - | [[Category: Padavannil, A | + | [[Category: Padavannil, A]] |
| - | [[Category: Sivaraman, J | + | [[Category: Sivaraman, J]] |
[[Category: Beta barrel]] | [[Category: Beta barrel]] | ||
[[Category: Hth motif]] | [[Category: Hth motif]] | ||
[[Category: Regulator]] | [[Category: Regulator]] | ||
[[Category: Transcription regulator]] | [[Category: Transcription regulator]] | ||
Revision as of 14:48, 21 December 2014
Structure of GrlR-GrlA complex
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