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3o0r

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{{STRUCTURE_3o0r| PDB=3o0r | SCENE= }}
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==Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment==
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===Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment===
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<StructureSection load='3o0r' size='340' side='right' caption='[[3o0r]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21109633}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3o0r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O0R FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_reductase_(cytochrome_c) Nitric-oxide reductase (cytochrome c)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.5 1.7.2.5] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o0r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o0r RCSB], [http://www.ebi.ac.uk/pdbsum/3o0r PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nitric oxide reductase (NOR) is an iron-containing enzyme that catalyzes the reduction of nitric oxide (NO) to generate a major greenhouse gas, nitrous oxide (N(2)O). Here, we report the crystal structure of NOR from Pseudomonas aeruginosa at 2.7 angstrom resolution. The structure reveals details of the catalytic binuclear center. The non-heme iron (Fe(B)) is coordinated by three His and one Glu ligands, but a His-Tyr covalent linkage common in cytochrome oxidases (COX) is absent. This structural characteristic is crucial for NOR reaction. Although the overall structure of NOR is closely related to COX, neither the D- nor K-proton pathway, which connect the COX active center to the intracellular space, was observed. Protons required for the NOR reaction are probably provided from the extracellular side.
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==Function==
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Structural basis of biological N2O generation by bacterial nitric oxide reductase.,Hino T, Matsumoto Y, Nagano S, Sugimoto H, Fukumori Y, Murata T, Iwata S, Shiro Y Science. 2010 Dec 17;330(6011):1666-70. Epub 2010 Nov 25. PMID:21109633<ref>PMID:21109633</ref>
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[[http://www.uniprot.org/uniprot/NORC_PSEAE NORC_PSEAE]] Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). [[http://www.uniprot.org/uniprot/NORB_PSEAE NORB_PSEAE]] Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen reduction is probably coupled to electron transport phosphorylation (By similarity).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3o0r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0R OCA].
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</div>
==See Also==
==See Also==
*[[Antibody|Antibody]]
*[[Antibody|Antibody]]
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*[[Nitric reductase|Nitric reductase]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:021109633</ref><references group="xtra"/><references/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Fukumori, Y.]]
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[[Category: Fukumori, Y]]
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[[Category: Hino, T.]]
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[[Category: Hino, T]]
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[[Category: Iwata, S.]]
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[[Category: Iwata, S]]
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[[Category: Matsumoto, Y.]]
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[[Category: Matsumoto, Y]]
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[[Category: Murata, T.]]
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[[Category: Murata, T]]
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[[Category: Nagano, S.]]
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[[Category: Nagano, S]]
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[[Category: Shiro, Y.]]
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[[Category: Shiro, Y]]
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[[Category: Sugimoto, H.]]
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[[Category: Sugimoto, H]]
[[Category: Cytoplasmic membrane]]
[[Category: Cytoplasmic membrane]]
[[Category: Electron transport]]
[[Category: Electron transport]]

Revision as of 06:42, 19 December 2014

Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment

3o0r, resolution 2.70Å

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