2p2r

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[[Image:2p2r.gif|left|200px]]<br /><applet load="2p2r" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2p2r.gif|left|200px]]
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caption="2p2r, resolution 1.60&Aring;" />
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'''Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA'''<br />
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{{Structure
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|PDB= 2p2r |SIZE=350|CAPTION= <scene name='initialview01'>2p2r</scene>, resolution 1.60&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CYT:6-AMINOPYRIMIDIN-2(1H)-ONE'>CYT</scene>
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|ACTIVITY=
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|GENE= PCBP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2P2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CYT:'>CYT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P2R OCA].
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2P2R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P2R OCA].
==Reference==
==Reference==
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Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution., Fenn S, Du Z, Lee JK, Tjhen R, Stroud RM, James TL, Nucleic Acids Res. 2007;35(8):2651-60. Epub 2007 Apr 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17426136 17426136]
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Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution., Fenn S, Du Z, Lee JK, Tjhen R, Stroud RM, James TL, Nucleic Acids Res. 2007;35(8):2651-60. Epub 2007 Apr 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17426136 17426136]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: protein-dna complex]]
[[Category: protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:25:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:07:49 2008''

Revision as of 16:07, 20 March 2008

Image:2p2r.gif


PDB ID 2p2r

Drag the structure with the mouse to rotate
, resolution 1.60Å
Ligands:
Gene: PCBP2 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA


Overview

KH (hnRNP K homology) domains, consisting of approximately 70 amino acid residues, are present in a variety of nucleic-acid-binding proteins. Among these are poly(C)-binding proteins (PCBPs), which are important regulators of mRNA stability and posttranscriptional regulation in general. All PCBPs contain three different KH domains and recognize poly(C)-sequences with high affinity and specificity. To reveal the molecular basis of poly(C)-sequence recognition, we have determined the crystal structure, at 1.6 A resolution, of PCBP2 KH3 domain in complex with a 7-nt DNA sequence (5'-AACCCTA-3') corresponding to one repeat of the C-rich strand of human telomeric DNA. The domain assumes a type-I KH fold in a betaalphaalphabetabetaalpha configuration. The protein-DNA interface could be studied in unprecedented detail and is made up of a series of direct and water-mediated hydrogen bonds between the protein and the DNA, revealing an especially dense network involving several structural water molecules for the last 2 nt in the core recognition sequence. Unlike published KH domain structures, the protein crystallizes without protein-protein contacts, yielding new insights into the dimerization properties of different KH domains. A nucleotide platform, an interesting feature found in some RNA molecules, was identified, evidently for the first time in DNA.

About this Structure

2P2R is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution., Fenn S, Du Z, Lee JK, Tjhen R, Stroud RM, James TL, Nucleic Acids Res. 2007;35(8):2651-60. Epub 2007 Apr 10. PMID:17426136

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