2pbd
From Proteopedia
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| - | [[Image:2pbd.jpg|left|200px]] | + | [[Image:2pbd.jpg|left|200px]] |
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| - | '''Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*''' | + | {{Structure |
| + | |PDB= 2pbd |SIZE=350|CAPTION= <scene name='initialview01'>2pbd</scene>, resolution 1.501Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= PFN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PBD is a [ | + | 2PBD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBD OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP., Ferron F, Rebowski G, Lee SH, Dominguez R, EMBO J. 2007 Oct 31;26(21):4597-606. Epub 2007 Oct 4. PMID:[http:// | + | Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP., Ferron F, Rebowski G, Lee SH, Dominguez R, EMBO J. 2007 Oct 31;26(21):4597-606. Epub 2007 Oct 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17914456 17914456] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
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[[Category: ternary complex; profilin; actin; vasp; poly-proline; loading poly-pro site; gab domain]] | [[Category: ternary complex; profilin; actin; vasp; poly-proline; loading poly-pro site; gab domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:11:07 2008'' |
Revision as of 16:11, 20 March 2008
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| , resolution 1.501Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | PFN1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*
Overview
Cells sustain high rates of actin filament elongation by maintaining a large pool of actin monomers above the critical concentration for polymerization. Profilin-actin complexes constitute the largest fraction of polymerization-competent actin monomers. Filament elongation factors such as Ena/VASP and formin catalyze the transition of profilin-actin from the cellular pool onto the barbed end of growing filaments. The molecular bases of this process are poorly understood. Here we present structural and energetic evidence for two consecutive steps of the elongation mechanism: the recruitment of profilin-actin by the last poly-Pro segment of vasodilator-stimulated phosphoprotein (VASP) and the binding of profilin-actin simultaneously to this poly-Pro and to the G-actin-binding (GAB) domain of VASP. The actin monomer bound at the GAB domain is proposed to be in position to join the barbed end of the growing filament concurrently with the release of profilin.
About this Structure
2PBD is a Protein complex structure of sequences from Homo sapiens and Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP., Ferron F, Rebowski G, Lee SH, Dominguez R, EMBO J. 2007 Oct 31;26(21):4597-606. Epub 2007 Oct 4. PMID:17914456
Page seeded by OCA on Thu Mar 20 18:11:07 2008
