2pd8
From Proteopedia
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| - | [[Image:2pd8.jpg|left|200px]] | + | [[Image:2pd8.jpg|left|200px]] |
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| - | '''1.8 Angstrom Crystal Structure of the Cys71Ser mutant of Vivid''' | + | {{Structure |
| + | |PDB= 2pd8 |SIZE=350|CAPTION= <scene name='initialview01'>2pd8</scene>, resolution 1.800Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= vvd, G17A4.050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Neurospora crassa]) | ||
| + | }} | ||
| + | |||
| + | '''1.8 Angstrom Crystal Structure of the Cys71Ser mutant of Vivid''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PD8 is a [ | + | 2PD8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PD8 OCA]. |
==Reference== | ==Reference== | ||
| - | Conformational switching in the fungal light sensor Vivid., Zoltowski BD, Schwerdtfeger C, Widom J, Loros JJ, Bilwes AM, Dunlap JC, Crane BR, Science. 2007 May 18;316(5827):1054-7. PMID:[http:// | + | Conformational switching in the fungal light sensor Vivid., Zoltowski BD, Schwerdtfeger C, Widom J, Loros JJ, Bilwes AM, Dunlap JC, Crane BR, Science. 2007 May 18;316(5827):1054-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17510367 17510367] |
[[Category: Neurospora crassa]] | [[Category: Neurospora crassa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lov domain; flavin; photoreceptor; circadian clock]] | [[Category: lov domain; flavin; photoreceptor; circadian clock]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:11:43 2008'' |
Revision as of 16:11, 20 March 2008
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| , resolution 1.800Å | |||||||
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| Ligands: | |||||||
| Gene: | vvd, G17A4.050 (Neurospora crassa) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
1.8 Angstrom Crystal Structure of the Cys71Ser mutant of Vivid
Overview
The Neurospora crassa photoreceptor Vivid tunes blue-light responses and modulates gating of the circadian clock. Crystal structures of dark-state and light-state Vivid reveal a light, oxygen, or voltage Per-Arnt-Sim domain with an unusual N-terminal cap region and a loop insertion that accommodates the flavin cofactor. Photoinduced formation of a cystein-flavin adduct drives flavin protonation to induce an N-terminal conformational change. A cysteine-to-serine substitution remote from the flavin adenine dinucleotide binding site decouples conformational switching from the flavin photocycle and prevents Vivid from sending signals in Neurospora. Key elements of this activation mechanism are conserved by other photosensors such as White Collar-1, ZEITLUPE, ENVOY, and flavin-binding, kelch repeat, F-BOX 1 (FKF1).
About this Structure
2PD8 is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.
Reference
Conformational switching in the fungal light sensor Vivid., Zoltowski BD, Schwerdtfeger C, Widom J, Loros JJ, Bilwes AM, Dunlap JC, Crane BR, Science. 2007 May 18;316(5827):1054-7. PMID:17510367
Page seeded by OCA on Thu Mar 20 18:11:43 2008
