2pff
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2pff.jpg|left|200px]] | + | [[Image:2pff.jpg|left|200px]] |
| - | + | ||
| - | '''Structural Insights of Yeast Fatty Acid Synthase''' | + | {{Structure |
| + | |PDB= 2pff |SIZE=350|CAPTION= <scene name='initialview01'>2pff</scene>, resolution 4.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Fatty-acyl-CoA_synthase Fatty-acyl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.86 2.3.1.86] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structural Insights of Yeast Fatty Acid Synthase''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2PFF is a [ | + | 2PFF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFF OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together., Lomakin IB, Xiong Y, Steitz TA, Cell. 2007 Apr 20;129(2):319-32. PMID:[http:// | + | The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together., Lomakin IB, Xiong Y, Steitz TA, Cell. 2007 Apr 20;129(2):319-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17448991 17448991] |
[[Category: Fatty-acyl-CoA synthase]] | [[Category: Fatty-acyl-CoA synthase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 26: | Line 35: | ||
[[Category: malonyltransferase]] | [[Category: malonyltransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:12:23 2008'' |
Revision as of 16:12, 20 March 2008
| |||||||
| , resolution 4.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Fatty-acyl-CoA synthase, with EC number 2.3.1.86 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural Insights of Yeast Fatty Acid Synthase
Overview
In yeast, the whole metabolic pathway for making 16- and 18-carbon fatty acids is carried out by fatty acid synthase, a 2.6 megadalton molecular-weight macromolecular assembly containing six copies of all eight catalytic centers. We have determined its crystal structure, which illuminates how this enzyme is initially activated and then carries out multiple steps of synthesis in each of six sterically isolated reaction chambers. Six of the catalytic sites are in the wall of the assembly facing an acyl carrier protein (ACP) bound to the ketoacyl synthase domain. Two-dimensional diffusion of substrates to the catalytic sites may be achieved by the electrostatically negative ACP swinging to each of the six electrostatically positive catalytic sites. The phosphopantetheinyl transferase domain lies outside the shell of the assembly, inaccessible to ACP that lies inside, suggesting that the attachment of the pantetheine arm to ACP must occur before complete assembly of the complex.
About this Structure
2PFF is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together., Lomakin IB, Xiong Y, Steitz TA, Cell. 2007 Apr 20;129(2):319-32. PMID:17448991[[Category: enoyl-[acyl-carrier-protein] reductase]]
Page seeded by OCA on Thu Mar 20 18:12:23 2008
