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3ab0

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{{STRUCTURE_3ab0| PDB=3ab0 | SCENE= }}
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==Crystal structure of complex of the Bacillus anthracis major spore surface protein BclA with ScFv antibody fragment==
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===Crystal structure of complex of the Bacillus anthracis major spore surface protein BclA with ScFv antibody fragment===
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<StructureSection load='3ab0' size='340' side='right' caption='[[3ab0]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21322055}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ab0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_str._'34f2_(nmrc)' Bacillus anthracis str. '34f2 (nmrc)'] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AB0 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2z5w|2z5w]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bclA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=526966 Bacillus anthracis str. '34F2 (NMRC)']), 35G-5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ab0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ab0 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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One method of laboratory- or field-based testing for anthrax is detection of Bacillus anthracis spores by high-affinity, high specificity binding reagents. From a pool of monoclonal antibodies, we selected one such candidate (A4D11) with high affinity for tBclA, a truncated version of the B. anthracis exosporium protein BclA. Kinetic analysis utilising both standard and kinetic titration on a Biacore biosensor indicated antibody affinities in the 300 pM range for recombinant tBclA, and the A4D11 antibody was also re-formatted into scFv configuration with no loss of affinity. However, assays against B. anthracis and related Bacilli species showed limited binding of intact spores as well as significant cross-reactivity between species. These results were rationalized by determination of the three-dimensional crystallographic structure of the scFv-tBclA complex. A4D11 binds the side of the tBclA trimer, contacting a face of the antigen normally packed against adjacent trimers within the exosporium structure; this inter-spore interface is highly conserved between Bacilli species. Our results indicate the difficulty of generating a high-affinity antibody to differentiate between the highly conserved spore structures of closely related species, but suggest the possibility of future structure-based antibody design for this difficult target.
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==About this Structure==
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Isolation, kinetic analysis, and structural characterization of an antibody targeting the Bacillus anthracis major spore surface protein BclA.,Nuttall SD, Wilkins ML, Streltsov VA, Pontes-Braz L, Dolezal O, Tran H, Liu CQ Proteins. 2011 Apr;79(4):1306-17. doi: 10.1002/prot.22971. Epub 2011 Feb 14. PMID:21322055<ref>PMID:21322055</ref>
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[[3ab0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_str._'34f2_(nmrc)' Bacillus anthracis str. '34f2 (nmrc)'] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AB0 OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
==See Also==
==See Also==
*[[Antibody|Antibody]]
*[[Antibody|Antibody]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021322055</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Streltsov, V A.]]
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[[Category: Streltsov, V A]]
[[Category: Anthrax]]
[[Category: Anthrax]]
[[Category: Exosporium]]
[[Category: Exosporium]]

Revision as of 14:14, 18 December 2014

Crystal structure of complex of the Bacillus anthracis major spore surface protein BclA with ScFv antibody fragment

3ab0, resolution 3.09Å

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