2pi0
From Proteopedia
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| - | [[Image:2pi0.gif|left|200px]] | + | [[Image:2pi0.gif|left|200px]] |
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| - | '''Crystal Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-B enhancer''' | + | {{Structure |
| + | |PDB= 2pi0 |SIZE=350|CAPTION= <scene name='initialview01'>2pi0</scene>, resolution 2.31Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= IRF3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-B enhancer''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PI0 is a [ | + | 2PI0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PI0 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-beta enhancer., Escalante CR, Nistal-Villan E, Shen L, Garcia-Sastre A, Aggarwal AK, Mol Cell. 2007 Jun 8;26(5):703-16. PMID:[http:// | + | Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-beta enhancer., Escalante CR, Nistal-Villan E, Shen L, Garcia-Sastre A, Aggarwal AK, Mol Cell. 2007 Jun 8;26(5):703-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17560375 17560375] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transcription activator/dna complex]] | [[Category: transcription activator/dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:13:12 2008'' |
Revision as of 16:13, 20 March 2008
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| , resolution 2.31Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | IRF3 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-B enhancer
Overview
Interferon regulatory factor 3 (IRF-3) is a key transcription factor in the assembly of the mammalian interferon-beta (IFN-beta) enhanceosome. We present here the structure of IRF-3 DNA binding domain in complex with the complete PRDIII-I regulatory element of the human IFN-beta enhancer. We show that four IRF-3 molecules bind in tandem to, variably spaced, consensus and nonconsensus IRF sites on the composite element. The ability of IRF-3 to bind these variable sites derives in part from two nonconserved arginines (Arg78 and Arg86) that partake in alternate protein-DNA contacts. We also show that the protein-DNA contacts are highly overlapped and that all four IRF sites are required for gene activation in vivo. In addition, we show that changing the nonconsensus IRF sites to consensus sites creates a more efficient enhancer in vivo. Together, the structure and accompanying biological data provide insights into the assembly of the IFN-beta enhanceosome in mammals.
About this Structure
2PI0 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-beta enhancer., Escalante CR, Nistal-Villan E, Shen L, Garcia-Sastre A, Aggarwal AK, Mol Cell. 2007 Jun 8;26(5):703-16. PMID:17560375
Page seeded by OCA on Thu Mar 20 18:13:12 2008
