2pt7
From Proteopedia
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| - | [[Image:2pt7.jpg|left|200px]] | + | [[Image:2pt7.jpg|left|200px]] |
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| - | '''Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)''' | + | {{Structure |
| + | |PDB= 2pt7 |SIZE=350|CAPTION= <scene name='initialview01'>2pt7</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= cag-alfa, cag-alpha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=210 Helicobacter pylori]), HP1451 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=210 Helicobacter pylori]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PT7 is a [ | + | 2PT7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PT7 OCA]. |
==Reference== | ==Reference== | ||
| - | Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase., Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G, EMBO J. 2007 Nov 28;26(23):4926-34. Epub 2007 Nov 1. PMID:[http:// | + | Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase., Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G, EMBO J. 2007 Nov 28;26(23):4926-34. Epub 2007 Nov 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17972918 17972918] |
[[Category: Helicobacter pylori]] | [[Category: Helicobacter pylori]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: type iv secretion]] | [[Category: type iv secretion]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:17:04 2008'' |
Revision as of 16:17, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | cag-alfa, cag-alpha (Helicobacter pylori), HP1451 (Helicobacter pylori) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)
Overview
Helicobacter pylori is one of the world's most successful human pathogens causing gastric ulcers and cancers. A key virulence factor of H. pylori is the Cag pathogenicity island, which encodes a type IV secretion system. HP0525 is an essential component of the Cag system and acts as an inner membrane associated ATPase. HP0525 forms double hexameric ring structures, with the C-terminal domains (CTDs) forming a closed ring and the N-terminal domains (NTDs) forming a dynamic, open ring. Here, the crystal structure of HP0525 in complex with a fragment of HP1451, a protein of previously unknown function, is reported. The HP1451 construct consists of two domains similar to nucleic acid-binding domains. Two HP1451 molecules bind to the HP0525 NTDs on opposite sides of the hexamer, locking it in the closed form and forming a partial lid over the HP0525 chamber. From the structure, it is suggested that HP1451 acts as an inhibitory factor of HP0525 to regulate Cag-mediated secretion, a suggestion confirmed by results of in vitro ATPase assay and in vivo pull-down experiments.
About this Structure
2PT7 is a Protein complex structure of sequences from Helicobacter pylori. Full crystallographic information is available from OCA.
Reference
Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase., Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G, EMBO J. 2007 Nov 28;26(23):4926-34. Epub 2007 Nov 1. PMID:17972918
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