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ATP-dependent DNA ligase from bacteriophage T7

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==Overview (General Function)==
==Overview (General Function)==
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ATP-dependent DNA ligase from bacteriophage T7 (Caudovirales Podoviridae) is used to catalyze a phosphodiester bond between single-strand nicks in double-stranded DNA. This occurs in replication (connecting okazaki fragments)<ref name="Berg">Berg, Jeremy M, Stryer, Lubert, Tymoczko, John L. ''Biochemistry.'' Sixth edition. New York: W.H. Freeman and Company, 2007: 796. Print.</ref>, DNA repair (excision repair), and recombination. DNA ligases require either ATP (eukaryotes and viruses) or NAD+ (prokaryotes) as a cofactor. All ligases require a divalent cation for function. Bacteriophage T7 DNA ligase uses Magnesium in vivo. A range of pH 7.2-7.7 is ideal for enzymatic activity. T7 ligase has a molecular weight of 41 kDa<ref>PMID: 8653795</ref>.
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ATP-dependent DNA ligase from bacteriophage T7 (Caudovirales Podoviridae<ref>McGrath S, van Sinderen D. Bacteriophage: ''Genetics and Molecular Biology.'' Norfolk: Caister Academic Press, 2007. Print.</ref>) is used to catalyze a phosphodiester bond between single-strand nicks in double-stranded DNA. This occurs in replication (connecting okazaki fragments)<ref name="Berg">Berg, Jeremy M, Stryer, Lubert, Tymoczko, John L. ''Biochemistry.'' Sixth edition. New York: W.H. Freeman and Company, 2007: 796. Print.</ref>, DNA repair (excision repair), and recombination. DNA ligases require either ATP (eukaryotes and viruses) or NAD+ (prokaryotes) as a cofactor. All ligases require a divalent cation for function. Bacteriophage T7 DNA ligase uses Magnesium in vivo. A range of pH 7.2-7.7 is ideal for enzymatic activity. T7 ligase has a molecular weight of 41 kDa<ref>PMID: 8653795</ref>.

Revision as of 03:38, 7 November 2013

ATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7

Structure of Bacteriophage T7 DNA Ligase(PDB entry 1a0i)

Drag the structure with the mouse to rotate


References

  1. ↑ McGrath S, van Sinderen D. Bacteriophage: Genetics and Molecular Biology. Norfolk: Caister Academic Press, 2007. Print.
  2. ↑ Berg, Jeremy M, Stryer, Lubert, Tymoczko, John L. Biochemistry. Sixth edition. New York: W.H. Freeman and Company, 2007: 796. Print.
  3. ↑ Subramanya HS, Doherty AJ, Ashford SR, Wigley DB. Crystal structure of an ATP-dependent DNA ligase from bacteriophage T7. Cell. 1996 May 17;85(4):607-15. PMID:8653795
  4. ↑ Doherty AJ, Ashford SR, Subramanya HS, Wigley DB. Bacteriophage T7 DNA ligase. Overexpression, purification, crystallization, and characterization. J Biol Chem. 1996 May 10;271(19):11083-9. PMID:8626651

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