2pwt
From Proteopedia
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| - | [[Image:2pwt.gif|left|200px]] | + | [[Image:2pwt.gif|left|200px]] |
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| - | '''Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group''' | + | {{Structure |
| + | |PDB= 2pwt |SIZE=350|CAPTION= <scene name='initialview01'>2pwt</scene>, resolution 1.800Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=LHA:DOUBLY FUNCTIONALIZED PAROMOMYCIN PM-II-162'>LHA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PWT is a [ | + | 2PWT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity., Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E, ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:[http:// | + | Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity., Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E, ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17722211 17722211] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Francois, B.]] | [[Category: Francois, B.]] | ||
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[[Category: aminoglycoside; haba group; ribosomal decoding site; x-ray analysis; rna]] | [[Category: aminoglycoside; haba group; ribosomal decoding site; x-ray analysis; rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:18:22 2008'' |
Revision as of 16:18, 20 March 2008
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| , resolution 1.800Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group
Overview
The crystal structure of the complex between oligonucleotide containing the bacterial ribosomal decoding site (A site) and the synthetic paromomycin analogue 1, which contains the gamma-amino-alpha-hydroxybutyryl (L-haba) group at position N1 of ring II (2-DOS ring), and an ether chain with an O-phenethylaminoethyl group at position C2 of ring III, is reported. Interestingly, next to the paromomycin analogue 1 specifically bound to the A site, a second molecule of 1 with a different conformation is observed at the crystal packing interface which mimics the A-minor interaction between two bulged-out adenines from the A site and the codon-anticodon stem of the mRNA-tRNA complex. Improved antibacterial activity supports the conclusion that analogue 1 might affect protein synthesis on the ribosome in two different ways: 1) specific binding to the A site forces maintenance of the "on" state with two bulged out adenines, and 2) a new binding mode of 1 to an A-minor motif which stabilizes complex formation between the ribosome and the mRNA-tRNA complex regardless of whether the codon-anticodon stem is of the cognate or near-cognate type.
About this Structure
2PWT is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity., Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E, ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:17722211
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