2lwl
From Proteopedia
(Difference between revisions)
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- | + | ==Structural Basis for the Interaction of Human β-Defensin 6 and Its Putative Chemokine Receptor CCR2 and Breast Cancer Microvesicles== | |
- | + | <StructureSection load='2lwl' size='340' side='right' caption='[[2lwl]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |
- | + | == Structural highlights == | |
+ | <table><tr><td colspan='2'>[[2lwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LWL FirstGlance]. <br> | ||
+ | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DEFB106A, BD6, DEFB106, DEFB6, DEFB106B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lwl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lwl RCSB], [http://www.ebi.ac.uk/pdbsum/2lwl PDBsum]</span></td></tr> | ||
+ | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Human beta-defensins (hBDs) are believed to function as alarm molecules that stimulate the adaptive immune system when a threat is present. In addition to its antimicrobial activity, defensins present other activities such as chemoattraction of a range of different cell types to the sites of inflammation. We have solved the structure of the hBD6 by NMR spectroscopy that contains a conserved beta-defensin domain followed by an extended C-terminus. We use NMR to monitor the interaction of hBD6 with microvesicles shed by breast cancer cell lines and with peptides derived from the extracellular domain of CC chemokine receptor 2 (Nt-CCR2) possessing or not possessing sulfation on Tyr26 and Tyr28. The NMR-derived model of the hBD6/CCR2 complex reveals a contiguous binding surface on hBD6, which comprises amino acid residues of the alpha-helix and beta2-beta3 loop. The microvesicle binding surface partially overlaps with the chemokine receptor interface. NMR spin relaxation suggests that free hBD6 and the hBD6/CCR2 complex exhibit microsecond-to-millisecond conformational dynamics encompassing the CCR2 binding site, which might facilitate selection of the molecular configuration optimal for binding. These data offer new insights into the structure-function relation of the hBD6-CCR2 interaction, which is a promising target for the design of novel anticancer agents. | ||
- | + | Structural Basis for the Interaction of Human beta-Defensin 6 and Its Putative Chemokine Receptor CCR2 and Breast Cancer Microvesicles.,De Paula VS, Gomes NS, Lima LG, Miyamoto CA, Monteiro RQ, Almeida FC, Valente AP J Mol Biol. 2013 Aug 11. pii: S0022-2836(13)00504-4. doi:, 10.1016/j.jmb.2013.08.001. PMID:23938203<ref>PMID:23938203</ref> | |
- | + | ||
- | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
- | + | </div> | |
+ | |||
+ | ==See Also== | ||
+ | *[[Defensin|Defensin]] | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
- | [[Category: Almeida, F C.L | + | [[Category: Almeida, F C.L]] |
- | [[Category: Gomes, N S.F | + | [[Category: Gomes, N S.F]] |
- | [[Category: Lima, L G | + | [[Category: Lima, L G]] |
- | [[Category: Miyamoto, C A | + | [[Category: Miyamoto, C A]] |
- | [[Category: Monteiro, R Q | + | [[Category: Monteiro, R Q]] |
- | [[Category: Paula, V S.de | + | [[Category: Paula, V S.de]] |
- | [[Category: Valente, A | + | [[Category: Valente, A]] |
[[Category: Antimicrobial protein]] | [[Category: Antimicrobial protein]] | ||
[[Category: Breast cancer]] | [[Category: Breast cancer]] | ||
[[Category: Dynamic]] | [[Category: Dynamic]] |
Revision as of 12:27, 18 December 2014
Structural Basis for the Interaction of Human β-Defensin 6 and Its Putative Chemokine Receptor CCR2 and Breast Cancer Microvesicles
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