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2yjt

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{{STRUCTURE_2yjt| PDB=2yjt | SCENE= }}
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==Crystal structure of E. coli DEAD-box protein SrmB bound to regulator of ribonuclease activity A (RraA)==
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===Crystal structure of E. coli DEAD-box protein SrmB bound to regulator of ribonuclease activity A (RraA)===
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<StructureSection load='2yjt' size='340' side='right' caption='[[2yjt]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24045937}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2yjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YJT FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yjv|2yjv]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjt RCSB], [http://www.ebi.ac.uk/pdbsum/2yjt PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Members of the DEAD-box family of RNA helicases contribute to virtually every aspect of RNA metabolism, in organisms from all domains of life. Many of these helicases are constituents of multi-component assemblies, and their interactions with partner proteins within the complexes underpin their activities and biological function. In Escherichia coli the DEAD-box helicase RhlB is a component of the multi-enzyme RNA degradosome assembly, and its interaction with the core ribonuclease RNase E boosts the ATP-dependent activity of the helicase (1,2). Earlier studies have identified the regulator of ribonuclease activity A (RraA) as a potential interaction partner of both RNase E and RhlB (3). We present structural and biochemical evidence showing how RraA can bind to, and modulate the activity of RhlB and another E. coli DEAD-box enzyme, SrmB. Crystallographic structures are presented of RraA in complex with a portion of the natively unstructured C-terminal tail of RhlB at 2.8 A resolution, and in complex with the C-terminal RecA-like domain of SrmB at 2.9 A. The models suggest two distinct mechanisms by which RraA might modulate the activity of these and potentially other helicases.
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==Function==
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Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases.,Pietras Z, Hardwick SW, Swiezewski S, Luisi BF J Biol Chem. 2013 Sep 17. PMID:24045937<ref>PMID:24045937</ref>
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[[http://www.uniprot.org/uniprot/D8AM26_ECOLX D8AM26_ECOLX]] Globally modulates RNA abundance by binding to RNase E (Rne) and regulating its endonucleolytic activity. Can modulate Rne action in a substrate-dependent manner by altering the composition of the degradosome. Modulates RNA-binding and helicase activities of the degradosome (By similarity).[HAMAP-Rule:MF_00471][SAAS:SAAS005493_004_044335] [[http://www.uniprot.org/uniprot/SRMB_ECOLI SRMB_ECOLI]] DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit at low temperature. Exhibits RNA-stimulated ATP hydrolysis and RNA unwinding activity. Acts before DeaD.<ref>PMID:12787353</ref> <ref>PMID:15196029</ref> <ref>PMID:15148362</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[2yjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJT OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:024045937</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Ecoli]]
[[Category: Ecoli]]
[[Category: RNA helicase]]
[[Category: RNA helicase]]
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[[Category: Hardwick, S W.]]
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[[Category: Hardwick, S W]]
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[[Category: Luisi, B F.]]
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[[Category: Luisi, B F]]
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[[Category: Pietras, Z.]]
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[[Category: Pietras, Z]]
[[Category: Dead box rna helicase]]
[[Category: Dead box rna helicase]]
[[Category: Hydrolase inhibitor-hydrolase complex]]
[[Category: Hydrolase inhibitor-hydrolase complex]]

Revision as of 14:22, 18 December 2014

Crystal structure of E. coli DEAD-box protein SrmB bound to regulator of ribonuclease activity A (RraA)

2yjt, resolution 2.90Å

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