2qcp
From Proteopedia
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| - | [[Image:2qcp.jpg|left|200px]] | + | [[Image:2qcp.jpg|left|200px]] |
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| - | '''1.0 A Structure of CusF-Ag(I) residues 10-88 from Escherichia coli''' | + | {{Structure |
| + | |PDB= 2qcp |SIZE=350|CAPTION= <scene name='initialview01'>2qcp</scene>, resolution 1.000Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=AG:SILVER ION'>AG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= cusF, cusX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''1.0 A Structure of CusF-Ag(I) residues 10-88 from Escherichia coli''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QCP is a [ | + | 2QCP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCP OCA]. |
==Reference== | ==Reference== | ||
| - | Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy., Loftin IR, Franke S, Blackburn NJ, McEvoy MM, Protein Sci. 2007 Oct;16(10):2287-93. PMID:[http:// | + | Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy., Loftin IR, Franke S, Blackburn NJ, McEvoy MM, Protein Sci. 2007 Oct;16(10):2287-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17893365 17893365] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: silver-binding]] | [[Category: silver-binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:24:25 2008'' |
Revision as of 16:24, 20 March 2008
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| , resolution 1.000Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | cusF, cusX (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
1.0 A Structure of CusF-Ag(I) residues 10-88 from Escherichia coli
Overview
Elevated levels of copper or silver ions in the environment are an immediate threat to many organisms. Escherichia coli is able to resist the toxic effects of these ions through strictly limiting intracellular levels of Cu(I) and Ag(I). The CusCFBA system is one system in E. coli responsible for copper/silver tolerance. A key component of this system is the periplasmic copper/silver-binding protein, CusF. Here the X-ray structure and XAS data on the CusF-Ag(I) and CusF-Cu(I) complexes, respectively, are reported. In the CusF-Ag(I) structure, Ag(I) is coordinated by two methionines and a histidine, with a nearby tryptophan capping the metal site. EXAFS measurements on the CusF-Cu(I) complex show a similar environment for Cu(I). The arrangement of ligands effectively sequesters the metal from its periplasmic environment and thus may play a role in protecting the cell from the toxic ion.
About this Structure
2QCP is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy., Loftin IR, Franke S, Blackburn NJ, McEvoy MM, Protein Sci. 2007 Oct;16(10):2287-93. PMID:17893365
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