4bj3
From Proteopedia
(Difference between revisions)
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| - | + | ==Integrin alpha2 I domain E318W-collagen complex== | |
| - | === | + | <StructureSection load='4bj3' size='340' side='right' caption='[[4bj3]], [[Resolution|resolution]] 3.04Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4bj3]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BJ3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bj3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bj3 RCSB], [http://www.ebi.ac.uk/pdbsum/4bj3 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The GFOGER motif in collagens (O denotes hydroxyproline) represents a high-affinity binding site for all collagen-binding integrins. Other GxOGER motifs require integrin activation for maximal binding. The E318W mutant of the integrin alpha2beta1 I domain displays a relaxed collagen specificity, typical of an active state. E318W binds more strongly than the wild-type alpha2 I domain to GMOGER, and forms a 2:1 complex with a homotrimeric, collagen-like, GFOGER peptide. Crystal structure analysis of this complex reveals two E318W I domains, A and B, bound to a single triple helix. The E318W I domains are virtually identical to the collagen-bound wild-type I domain, suggesting that the E318W mutation activates the I domain by destabilising the unligated conformation. E318W I domain A interacts with two collagen chains similarly to wild-type I domain (high-affinity mode). E318W I domain B makes favourable interactions with only one collagen chain (low-affinity mode). This observation suggests that single GxOGER motifs in the heterotrimeric collagens V and IX may support binding of activated integrins. | ||
| - | + | An activating mutation reveals a second binding mode of the integrin alpha2 I domain to the GFOGER motif in collagens.,Carafoli F, Hamaia SW, Bihan D, Hohenester E, Farndale RW PLoS One. 2013 Jul 29;8(7):e69833. doi: 10.1371/journal.pone.0069833. Print 2013. PMID:23922814<ref>PMID:23922814</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Integrin|Integrin]] | |
| - | [[Category: Bihan, D | + | == References == |
| - | [[Category: Carafoli, F | + | <references/> |
| - | [[Category: Farndale, R W | + | __TOC__ |
| - | [[Category: Hamaia, S W | + | </StructureSection> |
| - | [[Category: Hohenester, E | + | [[Category: Human]] |
| + | [[Category: Bihan, D]] | ||
| + | [[Category: Carafoli, F]] | ||
| + | [[Category: Farndale, R W]] | ||
| + | [[Category: Hamaia, S W]] | ||
| + | [[Category: Hohenester, E]] | ||
[[Category: Cell adhesion]] | [[Category: Cell adhesion]] | ||
Revision as of 09:27, 21 December 2014
Integrin alpha2 I domain E318W-collagen complex
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