2qij
From Proteopedia
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| - | [[Image:2qij.jpg|left|200px]] | + | [[Image:2qij.jpg|left|200px]] |
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| - | '''Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.''' | + | {{Structure |
| + | |PDB= 2qij |SIZE=350|CAPTION= <scene name='initialview01'>2qij</scene>, resolution 8.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=106821 Hepatitis B virus subtype adw]) | ||
| + | }} | ||
| + | |||
| + | '''Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QIJ is a [ | + | 2QIJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_b_virus_subtype_adw Hepatitis b virus subtype adw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QIJ OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:[http:// | + | Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17671358 17671358] |
[[Category: Hepatitis b virus subtype adw]] | [[Category: Hepatitis b virus subtype adw]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: viral capsid protein]] | [[Category: viral capsid protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:26:20 2008'' |
Revision as of 16:26, 20 March 2008
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| , resolution 8.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | C (Hepatitis B virus subtype adw) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.
Overview
Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells.
About this Structure
2QIJ is a Single protein structure of sequence from Hepatitis b virus subtype adw. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:17671358
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