2ql6
From Proteopedia
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| - | [[Image:2ql6.gif|left|200px]] | + | [[Image:2ql6.gif|left|200px]] |
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| - | '''human nicotinamide riboside kinase (NRK1)''' | + | {{Structure |
| + | |PDB= 2ql6 |SIZE=350|CAPTION= <scene name='initialview01'>2ql6</scene>, resolution 2.700Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=TIZ:(1R)-1-[4-(AMINOCARBONYL)-1,3-THIAZOL-2-YL]-1,4-ANHYDRO-D-RIBITOL'>TIZ</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= NRK1, C9orf95 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''human nicotinamide riboside kinase (NRK1)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QL6 is a [ | + | 2QL6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QL6 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of human nicotinamide riboside kinase., Khan JA, Xiang S, Tong L, Structure. 2007 Aug;15(8):1005-13. PMID:[http:// | + | Crystal structure of human nicotinamide riboside kinase., Khan JA, Xiang S, Tong L, Structure. 2007 Aug;15(8):1005-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17698003 17698003] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:26:58 2008'' |
Revision as of 16:26, 20 March 2008
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| , resolution 2.700Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | NRK1, C9orf95 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
human nicotinamide riboside kinase (NRK1)
Overview
Nicotinamide riboside kinase (NRK) has an important role in the biosynthesis of NAD(+) as well as the activation of tiazofurin and other NR analogs for anticancer therapy. NRK belongs to the deoxynucleoside kinase and nucleoside monophosphate (NMP) kinase superfamily, although the degree of sequence conservation is very low. We report here the crystal structures of human NRK1 in a binary complex with the reaction product nicotinamide mononucleotide (NMN) at 1.5 A resolution and in a ternary complex with ADP and tiazofurin at 2.7 A resolution. The active site is located in a groove between the central parallel beta sheet core and the LID and NMP-binding domains. The hydroxyl groups on the ribose of NR are recognized by Asp56 and Arg129, and Asp36 is the general base of the enzyme. Mutation of residues in the active site can abolish the catalytic activity of the enzyme, confirming the structural observations.
About this Structure
2QL6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human nicotinamide riboside kinase., Khan JA, Xiang S, Tong L, Structure. 2007 Aug;15(8):1005-13. PMID:17698003
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