2qlv
From Proteopedia
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| - | [[Image:2qlv.jpg|left|200px]] | + | [[Image:2qlv.jpg|left|200px]] |
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| - | '''Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1''' | + | {{Structure |
| + | |PDB= 2qlv |SIZE=350|CAPTION= <scene name='initialview01'>2qlv</scene>, resolution 2.600Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] | ||
| + | |GENE= SNF1, CAT1, CCR1, GLC2, PAS14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SIP2, SPM2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SNF4, CAT3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QLV is a [ | + | 2QLV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QLV OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1., Amodeo GA, Rudolph MJ, Tong L, Nature. 2007 Sep 27;449(7161):492-5. Epub 2007 Sep 12. PMID:[http:// | + | Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1., Amodeo GA, Rudolph MJ, Tong L, Nature. 2007 Sep 27;449(7161):492-5. Epub 2007 Sep 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17851534 17851534] |
[[Category: Non-specific serine/threonine protein kinase]] | [[Category: Non-specific serine/threonine protein kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transferase/protein binding complex]] | [[Category: transferase/protein binding complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:27:08 2008'' |
Revision as of 16:27, 20 March 2008
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| , resolution 2.600Å | |||||||
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| Gene: | SNF1, CAT1, CCR1, GLC2, PAS14 (Saccharomyces cerevisiae), SIP2, SPM2 (Saccharomyces cerevisiae), SNF4, CAT3 (Saccharomyces cerevisiae) | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1
Overview
AMP-activated protein kinase (AMPK) is a central regulator of energy homeostasis in mammals and is an attractive target for drug discovery against diabetes, obesity and other diseases. The AMPK homologue in Saccharomyces cerevisiae, known as SNF1, is essential for responses to glucose starvation as well as for other cellular processes, although SNF1 seems to be activated by a ligand other than AMP. Here we report the crystal structure at 2.6 A resolution of the heterotrimer core of SNF1. The ligand-binding site in the gamma-subunit (Snf4) has clear structural differences from that of the Schizosaccharomyces pombe enzyme, although our crystallographic data indicate that AMP can also bind to Snf4. The glycogen-binding domain in the beta-subunit (Sip2) interacts with Snf4 in the heterotrimer but should still be able to bind carbohydrates. Our structure is supported by a large body of biochemical and genetic data on this complex. Most significantly, the structure reveals that part of the regulatory sequence in the alpha-subunit (Snf1) is sequestered by Snf4, demonstrating a direct interaction between the alpha- and gamma-subunits and indicating that our structure may represent the heterotrimer core of SNF1 in its activated state.
About this Structure
2QLV is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1., Amodeo GA, Rudolph MJ, Tong L, Nature. 2007 Sep 27;449(7161):492-5. Epub 2007 Sep 12. PMID:17851534
Page seeded by OCA on Thu Mar 20 18:27:08 2008
Categories: Non-specific serine/threonine protein kinase | Protein complex | Saccharomyces cerevisiae | Amodeo, G A. | Rudolph, M J. | Tong, L. | Atp-binding | Carbohydrate metabolism | Cbs domain | Heterotrimer | Kinase | Lipoprotein | Membrane | Myristate | Nucleotide-binding | Nucleus | Phosphorylation | Serine/threonine-protein kinase | Transcription | Transcription regulation | Transferase | Transferase/protein binding complex
