2qsc

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[[Image:2qsc.jpg|left|200px]]<br /><applet load="2qsc" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2qsc.jpg|left|200px]]
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caption="2qsc, resolution 2.800&Aring;" />
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'''Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide'''<br />
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{{Structure
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|PDB= 2qsc |SIZE=350|CAPTION= <scene name='initialview01'>2qsc</scene>, resolution 2.800&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2QSC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QSC OCA].
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2QSC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QSC OCA].
==Reference==
==Reference==
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Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization., Bell CH, Pantophlet R, Schiefner A, Cavacini LA, Stanfield RL, Burton DR, Wilson IA, J Mol Biol. 2008 Jan 25;375(4):969-78. Epub 2007 Nov 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18068724 18068724]
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Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization., Bell CH, Pantophlet R, Schiefner A, Cavacini LA, Stanfield RL, Burton DR, Wilson IA, J Mol Biol. 2008 Jan 25;375(4):969-78. Epub 2007 Nov 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18068724 18068724]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: CL]]
[[Category: CL]]
[[Category: ZN]]
[[Category: ZN]]
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[[Category: aids]]
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[[Category: aid]]
[[Category: apoptosis]]
[[Category: apoptosis]]
[[Category: envelope protein]]
[[Category: envelope protein]]
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[[Category: virion]]
[[Category: virion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:29:03 2008''

Revision as of 16:29, 20 March 2008

Image:2qsc.jpg


PDB ID 2qsc

Drag the structure with the mouse to rotate
, resolution 2.800Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide


Overview

F425-B4e8 (B4e8) is a monoclonal antibody isolated from a human immunodeficiency virus type 1 (HIV-1)-infected individual that recognizes the V3 variable loop on the gp120 subunit of the viral envelope spike. B4e8 neutralizes a subset of HIV-1 primary isolates from subtypes B, C and D, which places this antibody among the very few human anti-V3 antibodies with notable cross-neutralizing activity. Here, the crystal structure of the B4e8 Fab' fragment in complex with a 24-mer V3 peptide (RP142) at 2.8 A resolution is described. The complex structure reveals that the antibody recognizes a novel V3 loop conformation, featuring a five-residue alpha-turn around the conserved GPGRA apex of the beta-hairpin loop. In agreement with previous mutagenesis analyses, the Fab' interacts primarily with V3 through side-chain contacts with just two residues, Ile(P309) and Arg(P315), while the remaining contacts are to the main chain. The structure helps explain how B4e8 can tolerate a certain degree of sequence variation within V3 and, hence, is able to neutralize an appreciable number of different HIV-1 isolates.

About this Structure

2QSC is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization., Bell CH, Pantophlet R, Schiefner A, Cavacini LA, Stanfield RL, Burton DR, Wilson IA, J Mol Biol. 2008 Jan 25;375(4):969-78. Epub 2007 Nov 13. PMID:18068724

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