This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2ahj
From Proteopedia
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2AHJ is a | + | 2AHJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis] with FE, ZN, SO4, NO and DIO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] Structure known Active Sites: 1, CTA and CTB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AHJ OCA]. |
==Reference== | ==Reference== | ||
| Line 36: | Line 36: | ||
[[Category: post-translational modification of cysteine residues]] | [[Category: post-translational modification of cysteine residues]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:04:36 2007'' |
Revision as of 11:59, 5 November 2007
|
NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE
Overview
The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme, that is inactivated in the dark because of persistent association with NO, and activated by photo-dissociation of NO. The crystal structure at 1.7 A, resolution and mass spectrometry revealed the structure of the non-heme, iron catalytic center in the nitrosylated state. Two Cys residues, coordinated to the iron were post-translationally modified to Cys-sulfenic, and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO, molecule. This unprecedented structure is likely to enable the, photo-regulation of NHase and will provide an excellent model for, designing photo-controllable chelate complexes and, ultimately, proteins.
About this Structure
2AHJ is a Protein complex structure of sequences from Rhodococcus erythropolis with FE, ZN, SO4, NO and DIO as ligands. Active as Nitrile hydratase, with EC number 4.2.1.84 Structure known Active Sites: 1, CTA and CTB. Full crystallographic information is available from OCA.
Reference
Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms., Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I, Nat Struct Biol. 1998 May;5(5):347-51. PMID:9586994
Page seeded by OCA on Mon Nov 5 14:04:36 2007
Categories: Nitrile hydratase | Protein complex | Rhodococcus erythropolis | Dohmae, N. | Endo, I. | Kamiya, N. | Nagashima, S. | Nakasako, M. | Odaka, M. | Takio, K. | Tsujimura, M. | Yohda, M. | DIO | FE | NO | SO4 | ZN | Hydratase | Lyase | Nitric oxide binding enzyme | Non-heme iron center | Photoreactive enzyme | Post-translational modification of cysteine residues
