2r33
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2r33.jpg|left|200px]] | + | [[Image:2r33.jpg|left|200px]] |
| - | + | ||
| - | '''Crystal structure of a Bowman-Birk inhibitor from Vigna unguiculata seeds''' | + | {{Structure |
| + | |PDB= 2r33 |SIZE=350|CAPTION= <scene name='initialview01'>2r33</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a Bowman-Birk inhibitor from Vigna unguiculata seeds''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2R33 is a [ | + | 2R33 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Vigna_unguiculata Vigna unguiculata]. This structure supersedes the now removed PDB entry 2OT6. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R33 OCA]. |
==Reference== | ==Reference== | ||
| - | Bowman-Birk protease inhibitor from the seeds of Vigna unguiculata forms a highly stable dimeric structure., Rao KN, Suresh CG, Biochim Biophys Acta. 2007 Oct;1774(10):1264-73. Epub 2007 Aug 3. PMID:[http:// | + | Bowman-Birk protease inhibitor from the seeds of Vigna unguiculata forms a highly stable dimeric structure., Rao KN, Suresh CG, Biochim Biophys Acta. 2007 Oct;1774(10):1264-73. Epub 2007 Aug 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17869196 17869196] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Vigna unguiculata]] | [[Category: Vigna unguiculata]] | ||
| Line 17: | Line 26: | ||
[[Category: bowman-birk protease inhibitor]] | [[Category: bowman-birk protease inhibitor]] | ||
[[Category: plant protein]] | [[Category: plant protein]] | ||
| - | [[Category: plant- | + | [[Category: plant-pi]] |
| - | [[Category: protein-protein | + | [[Category: protein-protein interaction]] |
[[Category: serine protease inhibitor]] | [[Category: serine protease inhibitor]] | ||
[[Category: vigna unguiculata]] | [[Category: vigna unguiculata]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:32:05 2008'' |
Revision as of 16:32, 20 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a Bowman-Birk inhibitor from Vigna unguiculata seeds
Overview
Different protease inhibitors including Bowman-Birk type (BBI) have been reported from the seeds of Vigna unguiculata. Protease isoinhibitors of double-headed Bowman-Birk type from the seeds of Vigna unguiculata have been purified and characterized. The BBI from Vigna unguiculata (Vu-BBI) has been found to undergo self-association to form very stable dimers and more complex oligomers, by size-exclusion chromatography and SDS-PAGE in the presence of urea. Many BBIs have been reported to undergo self-association to form homodimers or more complex oligomers in solution. Only one dimeric crystal structure of a BBI (pea-BBI) is reported to date. We report the three-dimensional structure of a Vu-BBI determined at 2.5 A resolution. Although, the inhibitor has a monomer fold similar to that found in other known structures of Bowman-Birk protease inhibitors, its quaternary structure is different from that commonly observed in this family. The structural elements responsible for the stability of monomer molecule and dimeric association are discussed. The Vu-BBI may use dimeric or higher quaternary association to maintain the physiological state and to execute its biological function.
About this Structure
2R33 is a Protein complex structure of sequences from Vigna unguiculata. This structure supersedes the now removed PDB entry 2OT6. Full crystallographic information is available from OCA.
Reference
Bowman-Birk protease inhibitor from the seeds of Vigna unguiculata forms a highly stable dimeric structure., Rao KN, Suresh CG, Biochim Biophys Acta. 2007 Oct;1774(10):1264-73. Epub 2007 Aug 3. PMID:17869196
Page seeded by OCA on Thu Mar 20 18:32:05 2008
