Sandbox Reserved 769

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<Structure load='3cep' size='500' frame='true' align='right' caption='Structure of a tryptophan synthase quinonoid intermediate.' />
<Structure load='3cep' size='500' frame='true' align='right' caption='Structure of a tryptophan synthase quinonoid intermediate.' />
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[[Image:TryptophanSynthase.png|250px|left|thumb| ]]
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[[Image:Tryptophan Synthase Dimer 3.png|250px|left|thumb| ]]
==Tryptophan Synthase==
==Tryptophan Synthase==

Revision as of 19:44, 6 December 2013

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This Sandbox is Reserved from Sep 25, 2013, through Mar 31, 2014 for use in the course "BCH455/555 Proteins and Molecular Mechanisms" taught by Michael B. Goshe at the North Carolina State University. This reservation includes Sandbox Reserved 299, Sandbox Reserved 300 and Sandbox Reserved 760 through Sandbox Reserved 779.
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Structure of a tryptophan synthase quinonoid intermediate.

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Tryptophan Synthase

Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae. However, it is absent from Animalia. [1]

Tryptophan synthase is a classic enzyme that channels a metabolic intermediate, indole

Contents


General Information

Gene Name: trpA, trpB[2]

Organism: Salmonella typhimurium (strain LT2) [2]

Classification: Lyase [3]

Length: trpA: 268 AA [2] trpB: 397 AA [2]

Molecular Weight: 72012.45 Da [4]

Isoelectric Point: 5.62 [5]

Chains: A, B [2]

Ligands: Sodium Ion ([1]), pyridoxal-5'-phosphate ([2])[3]


Structure

Hydrophobic channel: The α and β active sites are separated by a 25 angstrom long hydrophobic channel contained within the enzyme allowing for the diffusion of indole. If the channel did not exist, the indole formed at an α active site would quickly diffuse away and be lost to the cell as it is hydrophobic and can easily cross membranes. As such, the channel is essential for enzyme complex function[6]

Sequence of TrpA and Trp B

Alpha Subunit


Beta Subunit


Enzymatic Mechanism

References

  1. ↑ http://en.wikipedia.org/wiki/Tryptophan_synthase
  2. ↑ 2.0 2.1 2.2 2.3 2.4 http://www.uniprot.org/uniprot/P00929
  3. ↑ 3.0 3.1 http://oca.weizmann.ac.il/oca-bin/ocashort
  4. ↑ http://www.rcsb.org/pdb/explore.do?structureId=1QOP
  5. ↑ http://mips.helmholtz-muenchen.de/genre/proj/FGDB/singleGeneReport.html?entry=FGSG_10743
  6. ↑ http://www.annualreviews.org/doi/abs/10.1146/annurev.biochem.70.1.149
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