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3w8w
From Proteopedia
(Difference between revisions)
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| - | + | ==The crystal structure of EncM== | |
| - | ===The crystal structure of EncM | + | <StructureSection load='3w8w' size='340' side='right' caption='[[3w8w]], [[Resolution|resolution]] 1.95Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3w8w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'streptomyces_maritimus' 'streptomyces maritimus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W8W FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w8x|3w8x]], [[3w8y|3w8y]], [[3w8z|3w8z]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">encM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=115828 'Streptomyces maritimus'])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w8w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w8w RCSB], [http://www.ebi.ac.uk/pdbsum/3w8w PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Flavoproteins catalyse a diversity of fundamental redox reactions and are one of the most studied enzyme families. As monooxygenases, they are universally thought to control oxygenation by means of a peroxyflavin species that transfers a single atom of molecular oxygen to an organic substrate. Here we report that the bacterial flavoenzyme EncM catalyses the peroxyflavin-independent oxygenation-dehydrogenation dual oxidation of a highly reactive poly(beta-carbonyl). The crystal structure of EncM with bound substrate mimics and isotope labelling studies reveal previously unknown flavin redox biochemistry. We show that EncM maintains an unexpected stable flavin-oxygenating species, proposed to be a flavin-N5-oxide, to promote substrate oxidation and trigger a rare Favorskii-type rearrangement that is central to the biosynthesis of the antibiotic enterocin. This work provides new insight into the fine-tuning of the flavin cofactor in offsetting the innate reactivity of a polyketide substrate to direct its efficient electrocyclization. | ||
| - | + | Flavin-mediated dual oxidation controls an enzymatic Favorskii-type rearrangement.,Teufel R, Miyanaga A, Michaudel Q, Stull F, Louie G, Noel JP, Baran PS, Palfey B, Moore BS Nature. 2013 Nov 28;503(7477):552-6. doi: 10.1038/nature12643. Epub 2013 Oct 27. PMID:24162851<ref>PMID:24162851</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Streptomyces maritimus]] | [[Category: Streptomyces maritimus]] | ||
| - | [[Category: Baran, P S | + | [[Category: Baran, P S]] |
| - | [[Category: Louie, G | + | [[Category: Louie, G]] |
| - | [[Category: Michaudel, Q | + | [[Category: Michaudel, Q]] |
| - | [[Category: Miyanaga, A | + | [[Category: Miyanaga, A]] |
| - | [[Category: Moore, B S | + | [[Category: Moore, B S]] |
| - | [[Category: Noel, J P | + | [[Category: Noel, J P]] |
| - | [[Category: Palfey, B | + | [[Category: Palfey, B]] |
| - | [[Category: Stull, F | + | [[Category: Stull, F]] |
| - | [[Category: Teufel, R | + | [[Category: Teufel, R]] |
[[Category: Flavin binding]] | [[Category: Flavin binding]] | ||
[[Category: Monooxygenase]] | [[Category: Monooxygenase]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
Revision as of 18:03, 21 December 2014
The crystal structure of EncM
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