2rc3
From Proteopedia
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| - | [[Image:2rc3.jpg|left|200px]] | + | [[Image:2rc3.jpg|left|200px]] |
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| - | '''Crystal structure of CBS domain, NE2398''' | + | {{Structure |
| + | |PDB= 2rc3 |SIZE=350|CAPTION= <scene name='initialview01'>2rc3</scene>, resolution 1.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene> and <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= NE2398 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 Nitrosomonas europaea ATCC 19718]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of CBS domain, NE2398''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2RC3 is a [ | + | 2RC3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea_atcc_19718 Nitrosomonas europaea atcc 19718]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RC3 OCA]. |
==Reference== | ==Reference== | ||
| - | In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http:// | + | In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17982461 17982461] |
[[Category: Nitrosomonas europaea atcc 19718]] | [[Category: Nitrosomonas europaea atcc 19718]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: in situ proteolysis]] | [[Category: in situ proteolysis]] | ||
[[Category: mcsg]] | [[Category: mcsg]] | ||
| - | [[Category: midwest center for structural | + | [[Category: midwest center for structural genomic]] |
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi-2]] | [[Category: psi-2]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: unknown function]] | [[Category: unknown function]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:34:41 2008'' |
Revision as of 16:34, 20 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | NE2398 (Nitrosomonas europaea ATCC 19718) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of CBS domain, NE2398
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2RC3 is a Single protein structure of sequence from Nitrosomonas europaea atcc 19718. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Thu Mar 20 18:34:41 2008
Categories: Nitrosomonas europaea atcc 19718 | Single protein | Dong, A. | Edwards, A M. | Joachimiak, A. | Korniyenko, Y. | MCSG, Midwest Center for Structural Genomics. | Savchenko, A. | Walker, J R. | Xu, X. | Yakunin, A. | Zheng, H. | BR | NAD | Br | Cbs domain | In situ proteolysis | Mcsg | Midwest center for structural genomic | Protein structure initiative | Psi-2 | Structural genomic | Unknown function
