4mdt
From Proteopedia
(Difference between revisions)
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| - | + | ==Structure of LpxC bound to the reaction product UDP-(3-O-(R-3-hydroxymyristoyl))-glucosamine== | |
| - | + | <StructureSection load='4mdt' size='340' side='right' caption='[[4mdt]], [[Resolution|resolution]] 2.59Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4mdt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MDT FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=24G:URIDINE-5-DIPHOSPHATE-3-O-(R-3-HYDROXYMYRISTOYL)-GLUCOSAMINE'>24G</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpxC, O3O_04310 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mdt RCSB], [http://www.ebi.ac.uk/pdbsum/4mdt PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/K0BGQ2_ECO1E K0BGQ2_ECO1E]] Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell (By similarity).[HAMAP-Rule:MF_00388][SAAS:SAAS015870_004_013136] | [[http://www.uniprot.org/uniprot/K0BGQ2_ECO1E K0BGQ2_ECO1E]] Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell (By similarity).[HAMAP-Rule:MF_00388][SAAS:SAAS015870_004_013136] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The emergence of antibiotic-resistant strains of pathogenic bacteria is an increasing threat to global health that underscores an urgent need for an expanded antibacterial armamentarium. Gram-negative bacteria, such as Escherichia coli, have become increasingly important clinical pathogens with limited treatment options. This is due in part to their lipopolysaccharide (LPS) outer membrane components, which dually serve as endotoxins while also protecting Gram-negative bacteria from antibiotic entry. The LpxC enzyme catalyzes the committed step of LPS biosynthesis, making LpxC a promising target for new antibacterials. Here we present the first structure of an LpxC enzyme in complex with the deacetylation reaction product, UDP-(3-O-(R-3-hydroxymyristoyl))-glucosamine. These studies provide valuable insight into recognition of substrates and products by LpxC and a platform for structure-guided drug discovery of broad-spectrum Gram-negative antibiotics. | ||
| + | |||
| + | Structure of the bacterial deacetylase LpxC bound to the nucleotide reaction product reveals mechanisms of oxyanion stabilization and proton transfer.,Clayton GM, Klein DJ, Rickert KW, Patel SB, Kornienko M, Zugay-Murphy J, Reid JC, Tummala S, Sharma S, Singh SB, Miesel L, Lumb KJ, Soisson SM J Biol Chem. 2013 Oct 9. PMID:24108127<ref>PMID:24108127</ref> | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[UDP-3-O-acyl-N-acetylglucosamine deacetylase|UDP-3-O-acyl-N-acetylglucosamine deacetylase]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bacillus coli migula 1895]] | [[Category: Bacillus coli migula 1895]] | ||
| - | [[Category: Clayton, G M | + | [[Category: Clayton, G M]] |
| - | [[Category: Klein, D J | + | [[Category: Klein, D J]] |
| - | [[Category: Kornienko, M | + | [[Category: Kornienko, M]] |
| - | [[Category: Lumb, K J | + | [[Category: Lumb, K J]] |
| - | [[Category: Miesel, L | + | [[Category: Miesel, L]] |
| - | [[Category: Patel, S B | + | [[Category: Patel, S B]] |
| - | [[Category: Reid, J C | + | [[Category: Reid, J C]] |
| - | [[Category: Rickert, K W | + | [[Category: Rickert, K W]] |
| - | [[Category: Sharma, S | + | [[Category: Sharma, S]] |
| - | [[Category: Singh, S B | + | [[Category: Singh, S B]] |
| - | [[Category: Soisson, S M | + | [[Category: Soisson, S M]] |
| - | [[Category: Tummala, S | + | [[Category: Tummala, S]] |
| - | [[Category: Zugay-Murphy, J | + | [[Category: Zugay-Murphy, J]] |
[[Category: Deacetylase]] | [[Category: Deacetylase]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 21:10, 25 December 2014
Structure of LpxC bound to the reaction product UDP-(3-O-(R-3-hydroxymyristoyl))-glucosamine
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