2rfk

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[[Image:2rfk.jpg|left|200px]]<br /><applet load="2rfk" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2rfk.jpg|left|200px]]
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caption="2rfk, resolution 2.87&Aring;" />
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'''Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex'''<br />
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{{Structure
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|PDB= 2rfk |SIZE=350|CAPTION= <scene name='initialview01'>2rfk</scene>, resolution 2.87&Aring;
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|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+B+1'>AC1</scene>
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2RFK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+B+1'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFK OCA].
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2RFK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFK OCA].
==Reference==
==Reference==
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Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex., Liang B, Xue S, Terns RM, Terns MP, Li H, Nat Struct Mol Biol. 2007 Dec 2;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18059286 18059286]
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Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex., Liang B, Xue S, Terns RM, Terns MP, Li H, Nat Struct Mol Biol. 2007 Dec 2;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18059286 18059286]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: rrna processing]]
[[Category: rrna processing]]
[[Category: secsg]]
[[Category: secsg]]
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[[Category: southeast collaboratory for structural genomics]]
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[[Category: southeast collaboratory for structural genomic]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: trna processing]]
[[Category: trna processing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:35:37 2008''

Revision as of 16:35, 20 March 2008

Image:2rfk.jpg


PDB ID 2rfk

Drag the structure with the mouse to rotate
, resolution 2.87Å
Sites:
Ligands:
Coordinates: save as pdb, mmCIF, xml



Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex


Overview

The most complex RNA pseudouridylases are H/ACA ribonucleoprotein particles, which use a guide RNA for substrate capture and four proteins (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we report the three-dimensional structure of a catalytically deficient archaeal enzyme complex (including the guide RNA and three of the four essential proteins) bound to a substrate RNA. Extensive interactions of Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked guide-substrate RNA complex structure and position the substrate in proximity of the Cbf5 catalytic center. Our structural and complementary fluorescence analyses also indicate that precise placement of the target uridine at the active site requires a conformation of the guide-substrate RNA duplex that is brought about by the previously identified concurrent interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process.

About this Structure

2RFK is a Protein complex structure of sequences from Pyrococcus furiosus. Full crystallographic information is available from OCA.

Reference

Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex., Liang B, Xue S, Terns RM, Terns MP, Li H, Nat Struct Mol Biol. 2007 Dec 2;. PMID:18059286

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