4lhw
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Crystal structure of Rab8 in its active GppNHp-bound form== | |
| - | + | <StructureSection load='4lhw' size='340' side='right' caption='[[4lhw]], [[Resolution|resolution]] 1.55Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4lhw]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LHW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lhv|4lhv]], [[4lhx|4lhx]], [[4lhy|4lhy]], [[4lhz|4lhz]], [[4li0|4li0]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RAB8A, MEL, RAB8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lhw RCSB], [http://www.ebi.ac.uk/pdbsum/4lhw PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Small G-proteins (G) of the Ras-superfamily control the temporal and spatial coordination of intracellular signaling networks by acting as molecular on/off switches. Guanine nucleotide exchange factors (GEFs) regulate the activation of these G-proteins through catalytic replace-ment of guanosine diphosphate (GDP) by gua-nosine triphosphate (GTP). During nucleotide exchange, three distinct substrate-enzyme complexes occur: A ternary complex with GDP at the start of reaction (G:GDP:GEF), an intermediary nucleotide-free binary complex (G:GEF), and a ternary GTP-complex after productive G-protein activation (G:GTP:GEF). Here we show structural snapshots of the full nucleotide-exchange reaction sequence together with the G-protein substrates and products using Rabin8/GRAB (GEF) and Rab8 (G-protein) as a model system. Together with a thorough enzymatic characterization, our data provide a detailed view into the mechanism of Rabin8/GRAB mediated nucleotide exchange. | ||
| - | + | Intermediates in the Guanine Nucleotide Exchange Reaction of Rab8 Catalyzed by Rabin8/GRAB.,Guo Z, Hou X, Goody RS, Itzen A J Biol Chem. 2013 Sep 26. PMID:24072714<ref>PMID:24072714</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
| - | [[Category: Goody, R S | + | [[Category: Goody, R S]] |
| - | [[Category: Guo, Z | + | [[Category: Guo, Z]] |
| - | [[Category: Hou, X M | + | [[Category: Hou, X M]] |
| - | [[Category: Itzen, A | + | [[Category: Itzen, A]] |
[[Category: Protein transport]] | [[Category: Protein transport]] | ||
[[Category: Small gtpase]] | [[Category: Small gtpase]] | ||
Revision as of 17:57, 21 December 2014
Crystal structure of Rab8 in its active GppNHp-bound form
| |||||||||||
Categories: Human | Goody, R S | Guo, Z | Hou, X M | Itzen, A | Protein transport | Small gtpase
