2v1u
From Proteopedia
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| - | [[Image:2v1u.gif|left|200px]] | + | [[Image:2v1u.gif|left|200px]] |
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| - | '''STRUCTURE OF THE AEROPYRUM PERNIX ORC1 PROTEIN IN COMPLEX WITH DNA''' | + | {{Structure |
| + | |PDB= 2v1u |SIZE=350|CAPTION= <scene name='initialview01'>2v1u</scene>, resolution 3.10Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF THE AEROPYRUM PERNIX ORC1 PROTEIN IN COMPLEX WITH DNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V1U is a [ | + | 2V1U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V1U OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of DNA replication origin recognition by an ORC protein., Gaudier M, Schuwirth BS, Westcott SL, Wigley DB, Science. 2007 Aug 31;317(5842):1213-6. PMID:[http:// | + | Structural basis of DNA replication origin recognition by an ORC protein., Gaudier M, Schuwirth BS, Westcott SL, Wigley DB, Science. 2007 Aug 31;317(5842):1213-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17761880 17761880] |
[[Category: Aeropyrum pernix]] | [[Category: Aeropyrum pernix]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:42:25 2008'' |
Revision as of 16:42, 20 March 2008
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| , resolution 3.10Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE AEROPYRUM PERNIX ORC1 PROTEIN IN COMPLEX WITH DNA
Overview
DNA replication in archaea and in eukaryotes share many similarities. We report the structure of an archaeal origin recognition complex protein, ORC1, bound to an origin recognition box, a DNA sequence that is found in multiple copies at replication origins. DNA binding is mediated principally by a C-terminal winged helix domain that inserts deeply into the major and minor grooves, widening them both. However, additional DNA contacts are made with the N-terminal AAA+ domain, which inserts into the minor groove at a characteristic G-rich sequence, inducing a 35 degrees bend in the duplex and providing directionality to the binding site. Both contact regions also induce substantial unwinding of the DNA. The structure provides insight into the initial step in assembly of a replication origin and recruitment of minichromosome maintenance (MCM) helicase to that origin.
About this Structure
2V1U is a Protein complex structure of sequences from Aeropyrum pernix. Full crystallographic information is available from OCA.
Reference
Structural basis of DNA replication origin recognition by an ORC protein., Gaudier M, Schuwirth BS, Westcott SL, Wigley DB, Science. 2007 Aug 31;317(5842):1213-6. PMID:17761880
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