3wam

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{{STRUCTURE_3wam| PDB=3wam | SCENE= }}
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==Crystal structure of human LC3C_8-125==
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===Crystal structure of human LC3C_8-125===
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<StructureSection load='3wam' size='340' side='right' caption='[[3wam]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24290141}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3wam]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WAM FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vtu|3vtu]], [[3vtv|3vtv]], [[3vtw|3vtw]], [[3wal|3wal]], [[3wan|3wan]], [[3wao|3wao]], [[3wap|3wap]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MAP1LC3C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wam OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wam RCSB], [http://www.ebi.ac.uk/pdbsum/3wam PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Autophagy is a bulk degradation pathway that removes cytosolic materials to maintain cellular homeostasis. The autophagy-related gene 13 (Atg13) and microtubule associate protein 1 light chain 3 (LC3) proteins are required for autophagosome formation. We demonstrate that each of the human LC3 isoforms (LC3A, LC3B, and LC3C) interacts with Atg13 via the LC3 interacting region (LIR) of Atg13. Using X-ray crystallography, we solved the macromolecular structures of LC3A and LC3C, along with the complex structures of the LC3 isoforms with the Atg13 LIR. Together, our structural and binding analyses reveal that the side-chain of Lys49 of LC3 acts as a gatekeeper to regulate binding of the LIR. We verified this observation by mutation of Lys49 in LC3A, which significantly reduces LC3A positive puncta formation in cultured cells. Our results suggest that specific affinity of the LC3 isoforms to the Atg13 LIR is required for proper autophagosome formation.
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==Function==
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Structural Basis of the Autophagy-Related LC3/Atg13 LIR Complex: Recognition and Interaction Mechanism.,Suzuki H, Tabata K, Morita E, Kawasaki M, Kato R, Dobson RC, Yoshimori T, Wakatsuki S Structure. 2013 Nov 25. pii: S0969-2126(13)00401-2. doi:, 10.1016/j.str.2013.09.023. PMID:24290141<ref>PMID:24290141</ref>
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[[http://www.uniprot.org/uniprot/MLP3C_HUMAN MLP3C_HUMAN]] Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruites all ATG8 family members to infecting bacteria such as S.Typhimurium.<ref>PMID:23022382</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3wam]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WAM OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:024290141</ref><references group="xtra"/><references/>
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__TOC__
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[[Category: Dobson, R C.J.]]
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</StructureSection>
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[[Category: Kato, R.]]
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[[Category: Human]]
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[[Category: Kawasaki, M.]]
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[[Category: Dobson, R C.J]]
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[[Category: Morita, E.]]
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[[Category: Kato, R]]
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[[Category: Suzuki, H.]]
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[[Category: Kawasaki, M]]
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[[Category: Tabata, K.]]
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[[Category: Morita, E]]
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[[Category: Wakatsuki, S.]]
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[[Category: Suzuki, H]]
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[[Category: Yoshimori, T.]]
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[[Category: Tabata, K]]
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[[Category: Wakatsuki, S]]
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[[Category: Yoshimori, T]]
[[Category: Autophagy]]
[[Category: Autophagy]]
[[Category: Protein transport]]
[[Category: Protein transport]]
[[Category: Ubiquitin-like fold]]
[[Category: Ubiquitin-like fold]]

Revision as of 17:17, 21 December 2014

Crystal structure of human LC3C_8-125

3wam, resolution 1.75Å

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