2v3t

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[[Image:2v3t.jpg|left|200px]]<br /><applet load="2v3t" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2v3t.jpg|left|200px]]
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caption="2v3t, resolution 2.75&Aring;" />
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'''STRUCTURE OF THE LIGAND-BINDING CORE OF THE IONOTROPIC GLUTAMATE RECEPTOR-LIKE GLURDELTA2 IN THE APO FORM'''<br />
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{{Structure
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|PDB= 2v3t |SIZE=350|CAPTION= <scene name='initialview01'>2v3t</scene>, resolution 2.75&Aring;
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|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''STRUCTURE OF THE LIGAND-BINDING CORE OF THE IONOTROPIC GLUTAMATE RECEPTOR-LIKE GLURDELTA2 IN THE APO FORM'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2V3T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V3T OCA].
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2V3T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V3T OCA].
==Reference==
==Reference==
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Ionotropic glutamate-like receptor delta2 binds D-serine and glycine., Naur P, Hansen KB, Kristensen AS, Dravid SM, Pickering DS, Olsen L, Vestergaard B, Egebjerg J, Gajhede M, Traynelis SF, Kastrup JS, Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14116-21. Epub 2007 Aug 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17715062 17715062]
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Ionotropic glutamate-like receptor delta2 binds D-serine and glycine., Naur P, Hansen KB, Kristensen AS, Dravid SM, Pickering DS, Olsen L, Vestergaard B, Egebjerg J, Gajhede M, Traynelis SF, Kastrup JS, Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14116-21. Epub 2007 Aug 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17715062 17715062]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: ion transport]]
[[Category: ion transport]]
[[Category: ionic channel]]
[[Category: ionic channel]]
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[[Category: ionotropic glutamate receptors]]
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[[Category: ionotropic glutamate receptor]]
[[Category: ligand-binding core]]
[[Category: ligand-binding core]]
[[Category: membrane]]
[[Category: membrane]]
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[[Category: transport]]
[[Category: transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:53:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:42:58 2008''

Revision as of 16:42, 20 March 2008

Image:2v3t.jpg


PDB ID 2v3t

Drag the structure with the mouse to rotate
, resolution 2.75Å
Sites:
Ligands:
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE LIGAND-BINDING CORE OF THE IONOTROPIC GLUTAMATE RECEPTOR-LIKE GLURDELTA2 IN THE APO FORM


Overview

The orphan glutamate-like receptor GluRdelta2 is predominantly expressed in Purkinje cells of the central nervous system. The classification of GluRdelta2 to the ionotropic glutamate receptor family is based on sequence similarities, because GluRdelta2 does not form functional homomeric glutamate-gated ion channels in transfected cells. Studies in GluRdelta2(-/-) knockout mice as well as in mice with naturally occurring mutations in the GluRdelta2 gene have demonstrated an essential role of GluRdelta2 in cerebellar long-term depression, motor learning, motor coordination, and synaptogenesis. However, the lack of a known agonist has hampered investigations on the function of GluRdelta2. In this study, the ligand-binding core of GluRdelta2 (GluRdelta2-S1S2) was found to bind neutral amino acids such as D-serine and glycine, as demonstrated by isothermal titration calorimetry. Direct evidence for binding of D-serine and structural rearrangements in the binding cleft of GluRdelta2-S1S2 is provided by x-ray structures of GluRdelta2-S1S2 in its apo form and in complex with D-serine. Functionally, D-serine and glycine were shown to inactivate spontaneous ion-channel conductance in GluRdelta2 containing the lurcher mutation (EC(50) values, 182 and 507 microM, respectively). These data demonstrate that the GluRdelta2 ligand-binding core is capable of binding ligands and that cleft closure of the ligand-binding core can induce conformational changes that alter ion permeation.

About this Structure

2V3T is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Ionotropic glutamate-like receptor delta2 binds D-serine and glycine., Naur P, Hansen KB, Kristensen AS, Dravid SM, Pickering DS, Olsen L, Vestergaard B, Egebjerg J, Gajhede M, Traynelis SF, Kastrup JS, Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14116-21. Epub 2007 Aug 21. PMID:17715062

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