4k2i
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of ntda from bacillus subtilis with bound cofactor pmp== | |
| - | + | <StructureSection load='4k2i' size='340' side='right' caption='[[4k2i]], [[Resolution|resolution]] 2.23Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4k2i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K2I FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4k2b|4k2b]], [[4k2m|4k2m]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">939788, BSU10550, NP_388936.1, ntdA, yhjL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k2i RCSB], [http://www.ebi.ac.uk/pdbsum/4k2i PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/NTDA_BACSU NTDA_BACSU]] Part of the ntdABC operon that biosynthesizes the NTD (3,3'-neotrehalosadiamine) antibiotic. | [[http://www.uniprot.org/uniprot/NTDA_BACSU NTDA_BACSU]] Part of the ntdABC operon that biosynthesizes the NTD (3,3'-neotrehalosadiamine) antibiotic. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | NtdA from Bacillus subtilis is a sugar aminotransferase (SAT) that catalyses the PLP-dependent equatorial transamination of 3-oxo-alpha-D-glucose-6-phosphate to form alpha-D-kanosamine-6-phosphate. The crystal structure of NtdA shows that NtdA shares the common aspartate aminotransferase fold (AAT Type 1) with residues from both monomers forming the active site. The crystal structures of NtdA alone, co-crystallized with the product alpha-D-kanosamine-6-phosphate and incubated with the amine donor glutamate reveals three key structures in the mechanistic pathway of NtdA. The structure of NtdA alone reveals the internal aldimine form of NtdA with the cofactor PLP covalently attached to Lys247. Addition of glutamate results in formation of pyridoxamine phosphate. Co-crystallization with kanosamine-6-phosphate results in the formation of the external aldimine. Only alpha-D-kanosamine-6-phosphate is observed in the active site of NtdA, not the beta-anomer. A comparison of the structure and sequence of NtdA with other SATs enables us to propose that the VIbeta family of aminotransferases should be divided into subfamilies based on the catalytic lysine motif. | ||
| - | + | The structure of NtdA, a sugar aminotransferase involved in the kanosamine biosynthetic pathway in Bacillus subtilis, reveals a new sub-class of aminotransferases.,van Straaten KE, Ko JB, Jagdhane R, Anjum S, Palmer DR, Sanders DA J Biol Chem. 2013 Oct 4. PMID:24097983<ref>PMID:24097983</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bacsu]] | [[Category: Bacsu]] | ||
| - | [[Category: Palmer, D R.J | + | [[Category: Palmer, D R.J]] |
| - | [[Category: Sanders, D A.R | + | [[Category: Sanders, D A.R]] |
| - | [[Category: Straaten, K E.Van | + | [[Category: Straaten, K E.Van]] |
[[Category: Additional n-terninal domain]] | [[Category: Additional n-terninal domain]] | ||
[[Category: Aspertate aminotransferase fold]] | [[Category: Aspertate aminotransferase fold]] | ||
Revision as of 20:59, 25 December 2014
Crystal structure of ntda from bacillus subtilis with bound cofactor pmp
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