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== Introduction == | == Introduction == | ||
| + | <Structure load='2npu' size='300' frame='true' align='right' caption='Cartoon model of the FRB domain of mTOR' scene='56/568023/2npu_cartoon_rainbow/1' /> | ||
As a member of the phosphatidylinositol kinase-related kinases(PIKK)* the mammalian targert of rapamycin (mTOR)* | As a member of the phosphatidylinositol kinase-related kinases(PIKK)* the mammalian targert of rapamycin (mTOR)* | ||
is a multi domain protein which is involved in the regulation of cell growth and an important target of survival | is a multi domain protein which is involved in the regulation of cell growth and an important target of survival | ||
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Due to its inhibitory effect rapamycin has been a widely used tool for studying mTOR. | Due to its inhibitory effect rapamycin has been a widely used tool for studying mTOR. | ||
FRB is also capable of binding the activator phosphatidic acid* and small molecules, for example amino acids like | FRB is also capable of binding the activator phosphatidic acid* and small molecules, for example amino acids like | ||
| - | leucine. | + | leucine. |
| - | + | ||
{{clear}} | {{clear}} | ||
| - | == | + | == Structure == |
<Structure load='2npu' size='250' frame='true' align='right' caption='Insert caption here' scene='56/568023/2npu_cartoon_rainbow/1' /> | <Structure load='2npu' size='250' frame='true' align='right' caption='Insert caption here' scene='56/568023/2npu_cartoon_rainbow/1' /> | ||
And now let us try to implement a scence that highlights the <scene name='56/568023/Frb_domain_acidic_alpha1_4/1'>acidic residues</scene> of the alpha 1 and alpha 4 helices of the FRB domain | And now let us try to implement a scence that highlights the <scene name='56/568023/Frb_domain_acidic_alpha1_4/1'>acidic residues</scene> of the alpha 1 and alpha 4 helices of the FRB domain | ||
Revision as of 12:31, 1 January 2014
| This Sandbox is Reserved from 06/12/2018, through 30/06/2019 for use in the course "Structural Biology" taught by Bruno Kieffer at the University of Strasbourg, ESBS. This reservation includes Sandbox Reserved 1480 through Sandbox Reserved 1543. |
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FKBP12-rapamycin binding domain of mTOR
FRB domain of mTOR (or 2NPU) is responsible for the binding of the inhibitory cyclic macrolide Rapamycin
Introduction
|
As a member of the phosphatidylinositol kinase-related kinases(PIKK)* the mammalian targert of rapamycin (mTOR)* is a multi domain protein which is involved in the regulation of cell growth and an important target of survival signals. The sequence of 2549 residues is highly conserved across eukaryotes (40-60% precent sequence identity). The protein consists of several functional domains: At the N-terminus there are 12 HEAT* repeats followed by a central FAT* domain (residue 1513 - 1910), a FRB domain (residue 2015 - 2114) a serine-threonine kinase domain (residue 2181 - 2484) and a C-terminal FATC* domain (residue 2515 - 2549). The FKBP12-rapamycin binding (FRB) domain binds the inhibitory cyclic macroloide* rapamycin in complex with the small peptidyl-prolyl cis-trans isomerase FKBP12 leading to a decreased activity of the kinase domain. Due to its inhibitory effect rapamycin has been a widely used tool for studying mTOR. FRB is also capable of binding the activator phosphatidic acid* and small molecules, for example amino acids like leucine.
Structure
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And now let us try to implement a scence that highlights the of the alpha 1 and alpha 4 helices of the FRB domain
