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3j4u

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{{STRUCTURE_3j4u| PDB=3j4u | SCENE= }}
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==A new topology of the HK97-like fold revealed in Bordetella bacteriophage: non-covalent chainmail secured by jellyrolls==
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===A new topology of the HK97-like fold revealed in Bordetella bacteriophage: non-covalent chainmail secured by jellyrolls===
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<StructureSection load='3j4u' size='340' side='right' caption='[[3j4u]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24347545}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3j4u]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpbpp Bpbpp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J4U FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j4u RCSB], [http://www.ebi.ac.uk/pdbsum/3j4u PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteriophage BPP-1 infects and kills Bordetella species that cause whooping cough. Its diversity-generating retroelement (DGR) provides a naturally occurring phage-display system, but engineering efforts are hampered without atomic structures. Here, we report a cryo electron microscopy structure of the BPP-1 head at 3.5 A resolution. Our atomic model shows two of the three protein folds representing major viral lineages: jellyroll for its cement protein (CP) and HK97-like ('Johnson') for its major capsid protein (MCP). Strikingly, the fold topology of MCP is permuted non-circularly from the Johnson fold topology previously seen in viral and cellular proteins. We illustrate that the new topology is likely the only feasible alternative of the old topology. beta-sheet augmentation and electrostatic interactions contribute to the formation of non-covalent chainmail in BPP-1, unlike covalent inter-protein linkages of the HK97 chainmail. Despite these complex interactions, the termini of both CP and MCP are ideally positioned for DGR-based phage-display engineering. DOI: http://dx.doi.org/10.7554/eLife.01299.001.
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==About this Structure==
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A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 A resolution.,Zhang X, Guo H, Jin L, Czornyj E, Hodes A, Hui WH, Nieh AW, Miller JF, Zhou ZH Elife. 2013 Dec 17;2(0):e01299. doi: 10.7554/eLife.01299. PMID:24347545<ref>PMID:24347545</ref>
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[[3j4u]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_phage_bpp-1 Bordetella phage bpp-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4U OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024347545</ref><references group="xtra"/><references/>
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</div>
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[[Category: Bordetella phage bpp-1]]
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== References ==
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[[Category: Czornyj, E.]]
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<references/>
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[[Category: Guo, H.]]
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__TOC__
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[[Category: Hodes, A.]]
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</StructureSection>
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[[Category: Hui, W H.]]
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[[Category: Bpbpp]]
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[[Category: Jin, L.]]
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[[Category: Czornyj, E]]
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[[Category: Miller, J F.]]
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[[Category: Guo, H]]
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[[Category: Nieh, A W.]]
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[[Category: Hodes, A]]
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[[Category: Zhang, X.]]
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[[Category: Hui, W H]]
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[[Category: Zhou, Z H.]]
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[[Category: Jin, L]]
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[[Category: Miller, J F]]
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[[Category: Nieh, A W]]
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[[Category: Zhang, X]]
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[[Category: Zhou, Z H]]
[[Category: Cryoem]]
[[Category: Cryoem]]
[[Category: Protein topology]]
[[Category: Protein topology]]
[[Category: Virus]]
[[Category: Virus]]

Revision as of 21:32, 3 January 2015

A new topology of the HK97-like fold revealed in Bordetella bacteriophage: non-covalent chainmail secured by jellyrolls

3j4u, resolution 3.50Å

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