2z3j

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2z3j.gif|left|200px]]<br /><applet load="2z3j" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2z3j.gif|left|200px]]
-
caption="2z3j, resolution 1.60&Aring;" />
+
 
-
'''Crystal structure of blasticidin S deaminase (BSD) R90K mutant'''<br />
+
{{Structure
 +
|PDB= 2z3j |SIZE=350|CAPTION= <scene name='initialview01'>2z3j</scene>, resolution 1.60&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Blasticidin-S_deaminase Blasticidin-S deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.23 3.5.4.23]
 +
|GENE=
 +
}}
 +
 
 +
'''Crystal structure of blasticidin S deaminase (BSD) R90K mutant'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2Z3J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CAC:'>CAC</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Blasticidin-S_deaminase Blasticidin-S deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.23 3.5.4.23] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z3J OCA].
+
2Z3J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z3J OCA].
==Reference==
==Reference==
-
Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089374 10089374]
+
Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089374 10089374]
[[Category: Aspergillus terreus]]
[[Category: Aspergillus terreus]]
[[Category: Blasticidin-S deaminase]]
[[Category: Blasticidin-S deaminase]]
Line 30: Line 39:
[[Category: zinc]]
[[Category: zinc]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:59:57 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:51:46 2008''

Revision as of 16:51, 20 March 2008

Image:2z3j.gif


PDB ID 2z3j

Drag the structure with the mouse to rotate
, resolution 1.60Å
Ligands: , and
Activity: Blasticidin-S deaminase, with EC number 3.5.4.23
Coordinates: save as pdb, mmCIF, xml



Crystal structure of blasticidin S deaminase (BSD) R90K mutant


Overview

Blasticidin S deaminase from Aspergillus terreus was crystallized with polyethylene glycol 8000. Two types of crystals were grown under the same crystallization conditions. One type grew as thin plates, while the other had a rhombic shape. The rhombic shaped crystal was suitable for high-resolution crystal structure analysis. Precession photographs and diffraction data showed that the crystal belonged to orthorhombic space group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c = 56.48 A. The calculated Vm value was acceptable when a tetramer of the enzyme was contained in an asymmetric unit. Preliminary diffraction data were collected to a resolution of 2.0 A with good statistics.

About this Structure

2Z3J is a Single protein structure of sequence from Aspergillus terreus. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:10089374

Page seeded by OCA on Thu Mar 20 18:51:46 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2z3j"
Personal tools