3wnp

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'''Unreleased structure'''
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{{STRUCTURE_3wnp| PDB=3wnp | SCENE= }}
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===D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose===
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{{ABSTRACT_PUBMED_23908050}}
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The entry 3wnp is ON HOLD until Paper Publication
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==Function==
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[[http://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
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Authors: Suzuki, R., Suzuki, N., Fujimoto, Z., Momma, M., Kimura, K., Kitamura, S., Kimura, A., Funane, K.
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==About this Structure==
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[[3wnp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA].
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Description: D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose
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==Reference==
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<ref group="xtra">PMID:023908050</ref><references group="xtra"/><references/>
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[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
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[[Category: Fujimoto, Z.]]
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[[Category: Funane, K.]]
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[[Category: Kimura, A.]]
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[[Category: Kimura, K.]]
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[[Category: Kitamura, S.]]
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[[Category: Momma, M.]]
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[[Category: Suzuki, N.]]
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[[Category: Suzuki, R.]]
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[[Category: 6-glucan]]
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[[Category: Alpha-1]]
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[[Category: Beta-jelly roll]]
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[[Category: C2 type immunoglobulin fold]]
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[[Category: Glycoside hydrolase]]
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[[Category: Greek key]]
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[[Category: Transferase]]

Revision as of 11:13, 5 February 2014

Template:STRUCTURE 3wnp

Contents

D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose

Template:ABSTRACT PUBMED 23908050

Function

[CTA1_BACCI] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.[1]

About this Structure

3wnp is a 2 chain structure. Full crystallographic information is available from OCA.

Reference

  • Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K. Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):946-9. doi:, 10.1107/S174430911301991X. Epub 2013 Jul 27. PMID:23908050 doi:http://dx.doi.org/10.1107/S174430911301991X
  1. ↑ Oguma T, Tobe K, Kobayashi M. Purification and properties of a novel enzyme from Bacillus spp. T-3040, which catalyzes the conversion of dextran to cyclic isomaltooligosaccharides. FEBS Lett. 1994 May 30;345(2-3):135-8. PMID:7515357

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