2z8z
From Proteopedia
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| - | [[Image:2z8z.gif|left|200px]] | + | [[Image:2z8z.gif|left|200px]] |
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| - | '''Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase''' | + | {{Structure |
| + | |PDB= 2z8z |SIZE=350|CAPTION= <scene name='initialview01'>2z8z</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=PT:PLATINUM (II) ION'>PT</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2Z8Z is a [ | + | 2Z8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._mis38 Pseudomonas sp. mis38]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8Z OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S, FEBS Lett. 2007 Oct 30;581(26):5060-4. Epub 2007 Oct 1. PMID:[http:// | + | Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S, FEBS Lett. 2007 Oct 30;581(26):5060-4. Epub 2007 Oct 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17923123 17923123] |
[[Category: Pseudomonas sp. mis38]] | [[Category: Pseudomonas sp. mis38]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:52:58 2008'' |
Revision as of 16:53, 20 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | Triacylglycerol lipase, with EC number 3.1.1.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase
Overview
The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.
About this Structure
2Z8Z is a Single protein structure of sequence from Pseudomonas sp. mis38. Full crystallographic information is available from OCA.
Reference
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S, FEBS Lett. 2007 Oct 30;581(26):5060-4. Epub 2007 Oct 1. PMID:17923123
Page seeded by OCA on Thu Mar 20 18:52:58 2008
